Fundamentals of Recombinant Protein Production, Purification and Characterization

ISBN-13: 9780323983884 / Angielski / Miękka / 2024

Deepti Yadav; Abhishek Guldhe; Tukayi Kudanga

Opis

Fundamentals of Recombinant Protein Production, Purification and Characterization is organized into nine chapters in a logical fashion that cover an introduction to recombinant proteins and expression in different host expression systems, extraction, purification and analysis of proteins. This important reference features protocols, along with the advantages and disadvantage of each expression hosts and characterization technique (presented in tabular format) and offers detailed coverage of all aspects of protein production and processing (upstream and downstream processing) in one place. Finally, the book ends with different characterization techniques. Production of recombinant proteins for biotechnological and therapeutic applications at a large scale is an essential need of mankind. With the huge application potential of therapeutic and industrial proteins, there has been increasing demand for effective and efficient bioprocessing strategies. Recent progress around recombinant DNA technologies and bioprocessing strategies has paved the way for efficient production of recombinant proteins. Important factors such as insolubility and cost of production need to be considered for large scale production of these recombinant proteins.

Spis treści

1. Introduction to recombinant protein expression and purification
2. Recombinant protein expression in Escherichia coli
3. Recombinant protein expression in Pichia pastoris
4. Mammalian cells for recombinant protein production via transient gene transfer
5. Expression of recombinant proteins in Chlamydomonas reinhardtii
6. Extraction and concentration of proteins (Laboratory scale)
7. Chromatographic and non-chromatographic techniques for purifying recombinant proteins
8. Analysis for purity of recombinant proteins
9. Characterization of recombinant proteins