Fundamentals of Fluorescence Imaging

ISBN-13: 9789814774857 / Angielski / Twarda / 2019 / 458 str.

Guy Cox

Opis

Fluorescent indicators enable the imaging of dynamic changes in cell metabolism. This book covers for the first time imaging at all scales, from macro to sub-micro (super-resolution). All the contributors are at the cutting edge of their field.

Spis treści

What Is Fluorescence?; The Ploem Fluorescence Microscope; The Confocal Fluorescence Microscope; Multiphoton Fluorescence Microscopy; Immunofluorescence; Fluorescent Proteins; Dyes for Labeling Organelles and Compartments; Indicator and Reporter Dyes; New Approaches to Cancer Therapy Using In Vivo Fluorescent- Protein Imaging; Fluorescence Lifetime Imaging; Dynamic Experiments: FRAP and Photoconversion; Förster Resonant Energy Transfer (FRET); Recording the Fluorescent Image; Practical Aspects of Localisation Microscopy; Super-Resolution Optical Microscopy with Structured Illumination; STED Nanoscopy.