Functional Lipidomics

ISBN-13: 9781574444674 / Angielski / Twarda / 2005 / 352 str.

Li Feng; Glenn Prestwich; Feng Feng

Opis

Lipids play an essential role in cell signaling and subcellular structure. Systematic analysis of the total lipid structure of a cell or organism, the lipodome, can reveal novel avenues of therapeutic intervention and diagnosis. This analysis is best modeled after the lessons learned from proteomics. With contributions from pioneering researchers from the US, Europe, Canada, Japan, and China, Functional Lipidomics introduces lipidomics, explores the current state of the field, and provides a comprehensive background for future groundbreaking work in this burgeoning field. The first book dedicated to the topic, Functional Lipidomics first defines and introduces the field before delving deeply into the methods, processes, analyses, and research aspects. The book uncovers the biosynthesis, metabolism, and function of fatty acids, acylglyerols, glycerophospholipids, sphingolipids, terpenes, and lipids that complex with proteins. Taking both academic and industrial perspectives, the contributors elaborate upon cutting-edge approaches to lipid analysis, diagnostic approaches using lipid and lipid-binding protein patterns, and the therapeutic significance of targeting proteins involved with the lipid signaling pathway. Functional Lipidomics elucidates state-of-the-art strategies and techniques that enable us to understand biochemical patterns related to disease states, develop new targets for drug design, and identify important markers of a developmental stage, pathological condition, or disease treatment.

Spis treści

The LIPID MAPS Approach to Lipidomics. LC/MS Methodology in Lipid Analysis and Structural Characterization of Novel Lipid Species. Functional Plasticity of Lipid Mediators: The Example of Endocannabinoids. Eicosanoid Lipidomics. Functional Lipidomics: Lysophosphatidic Acid as a Target for Molecular Diagnosis and Therapy of Ovarian Cancer. Analysis of Lysophospholipids in Human Body Fluids: Comparison of the Lysophospholipid Content in Malignant vs. Nonmalignant Diseases. Functional Lipidomics: Lessons and Examples from the Sphingolipids. Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Analysis of Sphingolipids. Methods of Probing Phosphoinositides–Protein Interactions. Fishing for Pharmaceutically Relevant phosphoinositide-Binding Proteins Using Chemical Proteomics. Phosphoinositide Profiling in Complex Lipid Mixtures. Multiplexed Lipid Arrays of Antiimmunoglobulin M–Induced Changes in the Glycerophospholipid Composition of WEHI-231 Cells. Specific Lipid Alterations in Alzheimer’s Disease and Diabetes: Shotgun Global Cellular Lipidome Analyses by Electrospray Ionization Mass Spectrometry Using Intrasource Separation. High-Throughput Lipid Profiling to Identify and Characterize Genes Involved in Lipid Metabolism, Signaling, and Stress Response.

O autorze

Li Feng, Glenn D. Prestwich