Functional Genomics in Medicago Truncatula: Methods and Protocols

ISBN-13: 9781493993482 / Angielski / Miękka / 2019 / 343 str.

Luis A. Canas; Jose Pio Beltran

Spis treści

Chapter 1. Introduction

1.1. Legumes and agriculture sustainability

1.2. Grain and forage legumes: model systems used in developmental studies

1.3. Functional genomics in M. truncatula: applications in developmental studies

References

 

Chapter 2. M. truncatula genome sequencing: genomic data availability

Abstract

1. Introduction

2. The M. truncatula genome

3. Genomic data availability in M. truncatula

References

 

Chapter 3. Chemical mutagenesis in M. truncatula: ethyl methanesulphonate (EMS) and ethyl-nitrosourea (ENU)

Abstract

1. Introduction

2. Materials

3. Methods

4. Notes

References

 

Chapter 4. Physical mutagenesis in M. truncatula: g-rays and Fast Neutron Bombardment (FNB)

Abstract

1. Introduction

2. Materials

3. Methods

4. Notes

References

 

Chapter 5. T-DNA insertional mutagenesis and activation tagging in M. truncatula

Abstract

1. Introduction

2. Materials

3. Methods

4. Notes

References

 

Chapter 6. Tnt1 insertional mutagenesis in M. truncatula

Abstract

1. Introduction

2. Materials

3. Methods

4. Notes

References

 

Chapter 7. Target Induced Local Lesions IN Genomes (TILLING) in M. truncatula

Abstract

1. Introduction

2. Materials

3. Methods

4. Notes

References

 

Chapter 8. Transient post-transcriptional gene silencing in M. truncatula: Virus-Induced Gene Silencing (VIGS)

Abstract

1. Introduction

2. Materials

3. Methods

4. Notes

References

 

Chapter 9. Stable post-transcriptional gene silencing in M. truncatula: RNAi

Abstract

1. Introduction

2. Materials

3. Methods

4. Notes

References

 

Chapter 10. Gene silencing quantification in M. truncatula: PCR-based methods (semi- and quantitative RT-PCR)

Abstract

1. Introduction

2. Materials

3. Methods

4. Notes

References

 

Chapter 11. Gene silencing quantification in M. truncatula: fluorescence in situ
hibridization (FISH)

1. Introduction

2. Materials

3. Methods

4. Notes

References

Chapter 12. Functional genomics to study seed development in M. truncatula: an overview

Abstract

1. Introduction

2. Functional genomics and genetic control of seed development in M. truncatula

References

 

Chapter 13. Functional genomics to study plant architecture and branching in M. truncatula: an overview

Abstract

1. Introduction

2. Functional genomics and genetic control of plant architecture and branching in M. truncatula

References

 

Chapter 14. Functional genomics to study compound leaf development in M. truncatula: an overview

Abstract

1. Introduction

2. Functional genomics and genetic control of compound leaf development in M. truncatula

References

 

Chapter 15. Functional genomics and M. truncatula root and nodule development: an overview

Abstract

1. Introduction

2. Functional genomics and genetic control of root and nodule development in M. truncatula

References

 

Chapter 16. Functional genomics and M. truncatula flowering time: an overview

Abstract

1. Introduction

2. Functional genomics and genetic control of floral development in M. truncatula

References

 

Chapter 17. Functional genomics and M. truncatula floral development: an overview

Abstract

1. Introduction

2. Functional genomics and genetic control of floral development in M. truncatula

References

 

Chapter 18. Functional genomics and M. truncatula fruit development: an overview

Abstract

1. Introduction

2. Functional genomics and genetic control of fruit development in M. truncatula

References

 

Chapter 19. Emerging tools in functional genomics of M. truncatula: the CRISPR-Cas9 system

Abstract

1. Introduction

2. Editing the M. truncatula genome: targeted mutagenesis and gene editing using the CRISPR-Cas9 system

References

 

Chapter 20. Future prospects in functional genomics of legumes

1. Introduction

2. New legumes created through genome engineering versus transgenic ones carrying foreign DNA in their genomes: will be more acceptable for the consumers?

References

O autorze

Dr. Luis A. Cañas is a Staff Scientist of The Spanish National Research Council (CSIC) at the Institute for Plant Cell and Molecular Biology (IBMCP) of Valencia. While working in the Plant Molecular Biology Dep. at the University of Georgia (Athens, USA), he evaluated the use of floral-specific monoclonal antibodies as molecular markers to study floral organ development. On his return to Spain, he was firstly working at the Biological Research Center (CIB, CSIC) in Madrid and finally he became staff scientist of the CSIC at the IBMCP. Work in his lab is focused in the study of the MADS-box regulatory genes involved in floral organ development in legumes, using Medicago truncatula as model system and integrating molecular evolution approaches to determine their mode of evolution after duplication.

Dr. José Pío Beltrán is Professor of The Spanish National Research Council (CSIC) at the Institute for Plant Cell and Molecular Biology (IBMCP) of which he was the founder. While working at the Max Planck Institute für Züchtungsforschung (Köln, Germany) he isolated the gene Deficiens from Antirrhinum majus, the first floral organ identity homeotic gene to be discovered. He has been investigating for more than thirty years on floral and fruit development in legumes, especially in Medicago truncatula. He has been the President of the Federation of European Societies of Plant Biology (FESPB) as well as the President of the European Plant Science Organization (EPSO).

Blurb

This volume discusses popular methods to achieve different types of mutagenesis and forward/reverse genetics in Medicago truncatula. Several studies on genetic control of developmental and metabolic processes in this model legume are also described. The chapters in this book cover topics such as Targeting Induced Local Lesions IN Genomes (TILLING), Fast Neutron Bombardment (FNB), Tnt1 insertional mutagenesis, Virus-Induced Gene Silencing (VIGS), stable inactivation of microRNAs in roots, gene editing by CRISPR-Cas9, etc. This book also contains reviews on the specific use of these techniques in functional studies on the genetic control of seed, leaf, root, nodule, floral and fruit development in M. truncatula. Written for the highly successful Methods in Molecular Biology series format, chapters contain the kind of detailed description and implementation advice needed to promote success in the lab.

Cutting-edge and thorough, Functional Genomics in Medicago truncatula: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field.