Forensic DNA Typing Protocols

ISBN-13: 9781588292643 / Angielski / Twarda / 2004 / 280 str.

Angel Carracedo; Angel Carracedo

Opis

A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs.

Spis treści

Forensic DNA-Typing Technologies: A Review Angel Carracedo and Paula Sánchez-Diz DNA Extraction and Quantitation of Forensic Samples Using the Phenol-Chloroform Method and Real-Time PCR Silvano Köchl, Harald Niederstätter, and Walther Parson A Real-Time PCR Protocol to Determine the Number of Amelogenine (X-Y) Gene Copies From Forensic DNA Samples Antonio Alonso and Pablo Martín Species Determination: The Role and Use of the Cytochrome b Gene Adrian Linacre and James Chun-I Lee Constructing STR Mulitplex Assays John M. Butler Y Chromosome STR Typing Leonor Gusmão and Cíntia Alves Using Online Databases for Developing SNP Markers of Forensic Interest Christopher Phillips SNP Typing in Forensic Genetics: A Review Beatriz Sobrino and Angel Carracedo Genotyping SNPs With the LightCycler María Victoria Lareu and Clara Ruiz-Ponte Universal Tag Arrays in Forensic SNP Analysis Marie Allen and Anna-Maria Divne SNP Typing on the NanoChip Electronic Microarray Claus Børsting, Juan J. Sanchez, and Niels Morling Genotyping SNPs Using a UV-Photocleavable Oligonucleotide in MALDI-TOF MS Peter M. Vallone, Kristina Fahr, and Markus Kostrzewa Mitochondrial D-Loop and Coding Sequence Analysis Using Pyrosequencing Marie Allen and Hanna Andréasson SNaPshot Typing of Mitochondrial DNA Coding Region Variants Antonio Salas, Beatriz Quintáns, and Vanesa Álvarez-Iglesias Typing of Y Chromosome SNPs With Multiplex PCR Methods Juan J. Sanchez, Claus Børsting, and Niels Morling Y Chromosome SNP Analysis Using Single-Base Extension: A Hierarchical Multiplex Design María Brión SNaPshot for Pharmacogenetics by Minisequencing Klaus Bender Methods for the Study of Ancient DNA Christine Keyser-Tracqui and BertrandLudes Protocols for Ancient DNATyping Cristian Capelli and Frank Tschentscher Index

Blurb

In recent years, the rapid emergence of different markers and technologies for DNA typing has transformed the field of forensic identification with breathtaking speed. In Forensic DNA Typing Protocols, leading forensic geneticists from around the world describe in step-by-step detail the cutting-edge laboratory methods they now use for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. Additional chapters address new applications in the forensic genetics lab, such a species identification, ancient DNA, or typing of CYP polymorphisms for the analysis of adverse to drugs. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Up-to-date and highly practical, Forensic DNA Typing Protocols captures and makes practical for all laboratories the whole array of new technologies and techniques that have revolutionized forensic science.