Forensic DNA Analysis: Methods and Protocols

ISBN-13: 9781071632949 / Angielski

Catherine Connon

Opis

This volume focuses on the latest techniques used in forensic DNA analysis. The chapters include a comprehensive collection of extraction, quantification, STR amplification, and detection methods for routine forensic samples, including manual, semi-automated, and automated procedures using both home-brew and commercial products. The chapters also discuss probabilistic modeling software and specialized start-to-finish procedures for mitochondrial DNA analysis, archived latent fingerprints, latent DNA, rapid DNA profiling, and next-generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Forensic DNA Analysis: Methods and Protocols is a valuable resource for researchers interested in learning more about forensic DNA analysis procedures.

Spis treści

Preface…
Table of Contents…
Contributing Authors…

Part I Introduction

1. Forensic DNA Analysis: An Overview of the Laboratory Process
Catherine Cupples Connon

Part II DNA Extraction and Purification

2. Organic Extraction of Nucleic Acids using Ethanol Precipitation or Microcon® Centrifugal Filter Purification Methods
Carolyn Lewis

3. Manual Silica-Based DNA Extractions
Catherine Cupples Connon

4. Applied Biosystems™ PrepFiler™ Forensic DNA Extraction Kit (Manual and Semi-Automated via AutoMate Express™) 
Megan M. Foley

5. Robotic DNA Extraction Utilizing Qiagen BioSprint® 96 Workstation
Brittany Ziencik

6. DNA Extraction of Bone through Demineralization
Brandi L. Iorio and Ashley M. Cooley

7. Differential Extraction with Purification via Organic/Microcon® and Promega DNA IQ™ Methods
Jonathan Forsberg and Caitlin Ayoub

8. DNA Purification from Bloodstains and Buccal Cells/Saliva on FTA® Cards
Brittany C. Hudson and Catherine Cupples Connon

Part III DNA Quantification

9. Yield Gel via Quantitative Gel Electrophoresis
Victoria R. Parks and Dayanara A. Torres

10. Quantitative PCR of Alu Repeats using PowerUp™ SYBR® Green Master Mix
Sierra L. Laveroni and Victoria R. Parks

11. Quantitation of DNA using the Applied Biosystems Quantifiler® Trio DNA Quantification Kit
Kelly L. Knight, Angelina Mauriello, and Georgia Williams

12. QIAGEN's Investigator® Quantiplex Pro Kit 
Michelle D. Bonnette

Part IV STR Amplification

13. DNA Amplification using Promega's PowerPlex® Fusion Systems (5C and 6C)
Caitlin McCaughan and Kristy A. Lenz

14. Amplification of Extracted DNA and Direct Amplification with the PowerPlex® Y23 System 
Jonelle M. Thompson

15. Applied Biosystems' GlobalFiler™ PCR Amplification Kit
Georgia Williams, Megan M. Foley, and Kelly L. Knight

16. QIAGEN’s Investigator® 24plex QS and GO! PCR Amplification
Michelle D. Bonnette

17. Low Volume STR Amplification Options: Coupling with Standard or Fast PCR, Traditional or Normalized DNA Extraction, and/or Traditional or Alternative Capillary Electrophoresis
Catherine Cupples Connon

Part V STR Profile Detection and Interpretation

18. Capillary Electrophoresis with Applied Biosystems' 3500 Genetic Analyzer 
Kara Kovach

19. Likelihood Ratio Calculation using LRmix Studio
Megan M. Foley

Part VI Specialized Samples

20. Mitochondrial DNA Analysis
Ashley M. Cooley

21. An Optimized Forensic DNA Analysis Workflow for Obtaining STR Results from Archived Latent Fingerprints 
April D. Solomon

22. Detection of Latent DNA using a DNA Binding Dye
Adrian Linacre and Piyamas Petcharoen

23. Rapid DNA Profile Development with Applied Biosystems RapidHIT™ ID System 
Megan M. Foley

24. Next-Generation Sequencing: ForenSeq™ DNA Signature Prep Kit with the Illumina MiSeq FGx
Megan M. Foley

Subject Index List…