Fish Cytogenetic Techniques: Ray-Fin Fishes and Chondrichthyans

ISBN-13: 9780367377557 / Angielski / Miękka / 2019 / 216 str.

Catherine Ozouf-Costaz; Eva Pisano; Fausto Foresti

Opis

Traditional cytogenetic techniques and molecular cytogenetics have greatly improved, but technical details and protocols are often not published in scientific journals due to constraints in format and size, and therefore are not accessible to the whole scientific community. This book fills a need for a handbook that specifically deals with fish cytogenetics. It brings together and updates the main techniques to standardize traditional methods and share novel developments and improvements with the entire scientific community interested in fundamental and applied fish cytogenetics.

Spis treści

Teleost Fish Handling and Transport under Reduced Stress Conditions. Storage of Karyotyped Voucher Specimens and their Molecular Identification. Direct Chromosome Preparation from Freshwater Teleost Fishes. Direct Mitotic Chromosome Preparations from Chondrichthyan Tissues. Mitotic Chromosome Preparations of Freshwater Stingrays. Direct Chromosome Preparation from Regenerating Fish Fin Tissue. Direct Chromosome Preparations from Embryos and Larvae. Establishment of Sturgeon Primary Cell Lines. Teleost Fish Lymphocyte Culture. Rapid Fibroblast Culture for Teleost Fish Karyotyping. Cephalic Kidney and Spleen Cell Culture in Antarctic Teleosts. Meiotic Surface-spread and Immunodetection for Synaptonemal Complex Proteins in Teleost fish. C-banding. Sequential Chromosome Banding in Fishes. Standard Fluorescence in Situ Hybridization Procedures. Characterization of Fish Genomes by GISH and CGH. Fish Chromosome Microdissection and its Utilization in the Synthesis of Fluorescent in situ Hybridization Probes. Microdissection and Chromosome Painting. Extending DNA Fibers and Fiber-FISH. In vivo Micronucleus Test for Detecting Response of Teleost Fish to Environmental Genotoxic Exposure.

O autorze

Catherine Ozouf-Costaz, Eva Pisano, Fausto Foresti, Lurdes Foresti de Almeida Toledo