Familial Cardiomyopathies: Methods and Protocols

ISBN-13: 9781071635261 / Angielski

Michael Regnier; Matthew Childers

Spis treści

Preface…
Table of Contents…
Contributing Authors…

1. Echocardiography to Assess Cardiac Structure and Function in Genetic Cardiomyopathies
Galina Flint, Kristina Kooiker, and Farid Moussavi-Harami

2. MRI Quantification of Cardiac Structure and Function in Cardiomyopathy Patients
Anna Naumova, Xue-Qiao Zhao, and Chun Yuan

3. Atomistic Simulations of Sarcomere Proteins
Matthew Carter Childers and Michael Regnier

4. Design Principles and Benefits of Spatially Explicit Models of Myofilament Function
Bertrand C.W. Tanner

5. Computational Modeling of Cardiac Electrophysiology
Haibo Ni and Eleonora Grandi

6. Computational Modeling and Simulation of the Fibrotic Human Atria
Savannah F. Bifulco and Patrick M. Boyle

7. A Semi-Automatic Pipeline for Generation of Large Cohorts of Four-Chamber Heart Meshes
Marina Strocchi, Cristobal Rodero, Caroline Roney, Caroline Mendonca Costa, Gernot Plank, Pablo Lamata, and Steven A. Niederer

8. Genomic Engineering of Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Christopher Cavanaugh, Jennifer Hesson, and Julie Mathieu

9. The GENTIL Method for Isolation of Human Adult Cardiomyocytes from Cryopreserved Tissue for Proteomic Analyses
Michelle Waknitz, Linda Berg Luecke, Roneldine Mesidor, Melinda Wojtkiewicz, Chase Castro, and Rebekah L. Gundry

10. Motility Assay to Probe the Calcium Sensitivity of Myosin and Regulated Thin Filaments
Chao Liu, Kathleen M. Ruppel, and James A. Spudich

11. Protocols for Myosin and Actin-Myosin Assays using Rapid, Stopped-Flow Kinetics
Kerry Kao and Michael A. Geeves

12. Measuring the Contractile Kinetics of Isolated Myofibrils from Human-Induced Pluripotent Stem Cell Derived Cardiomyocyte (hiPSC-CM) Models of Cardiomyopathy
Saffie Mohran, Sonette Steczina, Christian Mandrycky, Kerry Kao, and Michael Regnier

Subject Index List…

Blurb

This volume covers the latest advances in technologies that look at familial cardiomyopathies in greater detail, and provides new computational and experimental models that model, study, and detect disease at earlier stages. Together, this allows interdisciplinary research experiments to provide new insights for the development of novel interventions that slow, stop, or even reverse the disease process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Familial Cardiomyopathies: Methods and Protocols aims to inspire further development of techniques used to study myocardial disease and the development of new, sarcomere-targeted therapeutic approaches for the maintenance of heart health and the treatment of heart failure.