Extracellular Vesicles in Health and Disease

ISBN-13: 9789814411981 / Angielski / Twarda / 2014 / 482 str.

Paul Harrison; Chris Gardiner; Ian L. Sargent

Opis

Interest in the role of extracellular vesicles (microvesicles and exosomes) is expanding rapidly. It is now apparent that far from being merely cellular debris, these vesicles play a key role in cell-to-cell communication and signaling. Moreover, they are significantly elevated in a number of diseases. This raises the question of their direct role in pathogenesis as well as their possible use as biomarkers. This book stems from the first international meeting on "Microvesicles and Nanovesicles in Health and Disease" held at Magdalen College, Oxford, in 2010. The purpose of the meeting was to bring together, for the first time, a range of experts from around the world to discuss the latest advances in this field. Key to the study of these vesicles is the availability of methodologies for their measurement in biological fluids. A major section of the meeting focused on a range of exciting new technologies which have been developed for this purpose. The presentations at this meeting form the basis of this book, which will appeal to basic scientists, clinicians, and those developing technology for the measurement of extracellular vesicles.

Spis treści

OVERVIEW. Overview of Extracellular Vesicles in Health and Disease. The Biogenesis of Exosomes. Extracellular Vesicle-Mediated Epigenetic Reprogramming of Cells. TECHNOLOGIES FOR MEASUREMENT OF EXTRACELLULAR VESICLES. An Overview of Novel and Conventional Methods to Detect Extracellular Vesicles. Standardization and Pre-Analytical Variables. Procoagulant Assays. Capture Based Assays for Extracellular Vesicles within the Blood. Flow Cytometry. Atomic Force Microscopy Measurement of Extracellular Vesicles Derived from Plasma. Light Scattering Methods to Characterize Extracellular Vesicles. Nanoparticle Tracking Analysis. Microvesicle and Exosome Proteomic Analysis – Extracellular Vesicle Proteomic Analysis. CLINICAL PERSPECTIVES. Microvesicles and Exosomes in Cancer. Extracellular Vesicles and Tissue Factor. Extracellular Vesicles in Normal Pregnancy and Pre-Eclampsia. Extracellular Vesicles in Cardiovascular Disease. Extracellular Vesicles in Immunology.