Extracellular Vesicles in Diagnosis and Therapy

ISBN-13: 9781071623435 / Angielski

Maurizio Federico; Barbara Ridolfi

Spis treści

Part I: Extracellular Vesicles Detection and Isolation

 

1. Aptasensors for Cancerous Exosome Detection

            Jin Li, Sitao Xie, Fengli Qu, and Weihong Tan

 

2. Detection of Cancer-Derived Exosomes Using a Sensitive Colorimetric Aptasensor

            Lizhou Xu and Khuloud T. Al-Jamal

 

3. Isolation of Circulating Extracellular Vesicles by High-Performance Size-Exclusion Chromatography

            Kaloyan Takov, I-Jou Teng, and Manuel Mayr

 

4. Isolation and Proteomic Analysis of Mouse Serum Small Extracellular Vesicles for Individual Subject Analysis

            Federica Anastasi, Marialaura Dilillo, Davide Pellegrini, and Liam A. McDonnell

 

5. Protocol for Measuring Concentrations of Extracellular Vesicles in Human Blood Plasma with Flow Cytometry

            Najat Hajji, Chi M. Hau, Rienk Nieuwland, and Edwin van der Pol

 

Part II: Isolation and Characterization of Tissue and Biofluid-Specific EVs

 

6. Targeted Mass Spectrometry-Based Proteomics Method to Quantify Placental Extracellular Vesicles

            Andrew Lai, Carlos Palma, Alexis Salas, Flavio Carrion, and Carlos Salomon

 

7. Isolation and Characterization of Extracellular Vesicles Secreted from Human Pluripotent Stem Cell-Derived Cardiovascular Progenitor Cells

            Qiang Wu, Min-Xia Ke, and Huang-Tian Yang

 

8. Isolation and Characterization of Salivary Exosomes for Cancer Biomarker Discovery

            Lucas Trevisan França de Lima, Juliana Müller Bark, Mohammad Rasheduzzaman, Chameera Ekanayake Weeramange, and Chamindie Punyadeera

 

9. Isolation and Characterization of Urinary Extracellular Vesicles for MicroRNA Biomarker Signature Development with Reference to MISEV Compliance

            Marlene Reithmair, Anja Lindemann, Veronika Mussack, and Michael W. Pfaffl

 

Part III: Analysis of EV Components

 

10. Direct Detection of Extracellular Vesicle miRNAs Using a Single-Step RT-qPCR Assay

            Ayyanar Sivanantham, Heedoo Lee, and Yang Jin

 

11. Purification and Phosphoproteomic Analysis of Plasma-Derived Extracellular Vesicles

            Anton B. Iliuk

 

12. Lipidomic Analysis of Extracellular Vesicles Isolated from Human Plasma and Serum

            Yuchen Sun, Kosuke Saito, and Yoshiro Saito

 

Part IV: EV Engineering

 

13. Extracellular Vesicles and their Use as Vehicles of Immunogens

            Chiara Chiozzini, Barbara Ridolfi, and Maurizio Federico

 

14. Isolation and Fluorescent Labeling of Extracellular Vesicles from Cultured Tumor Cells

            Noelle Leary, Sarina Walser, and Lothar C. Dieterich

 

15. Generation, Characterization, and Count of Fluorescent Extracellular Vesicles

            Flavia Ferrantelli, Valentina Tirelli, Valeria Barreca, and Francesco Manfredi

 

16. Optimized Protocol for Plasma-Derived Extracellular Vesicles Loading with Synthetic miRNA Mimic Using Electroporation

            Margherita A.C. Pomatto, Federica Negro, and Giovanni Camussi

 

17. Extracellular Vesicle Loading via pH-Gradient Modification

            Stephanie M. Kronstadt, Steven M. Jay, and Anjana Jeyaram

Blurb

This detailed book provides an exhaustive picture of current methods to detect, isolate, and analyze extracellular vesicles (EVs) from diverse sources, now seen as potential disease biomarkers as well as tools for the development of new therapies. Beginning with a section on detection and isolation of EVs, the volume continues with chapters covering different methods to isolate and quantify EVs from specialized tissues/organs and body fluids, methods devoted to analyzing EV components, as well as cutting-edge methods to engineer EVs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Extracellular Vesicles in Diagnosis and Therapy serves as an ideal guide for researchers seeking to learn more about the complexities of cell-to-cell communication.