Extracellular Matrix Protocols: Second Edition

ISBN-13: 9781617378676 / Angielski / Miękka / 2010 / 324 str.

Sharona Even-Ram; Vira Artym

Opis

The study of the extracellular matrix (ECM) and its diverse roles in tissue scaffolding and cellular signaling in both physiological and pathological processes has significantly expanded over the past decade. Although well appreciated, the structural and biochemical complexity and the dynamic nature of the living matrix are still under extensive inv- tigation, yielding a growing number of methods with varying degree of sophistication and intricacy. In this edition of Extracellular Matrix Protocols, we compiled a variety of methods that can be readily reproduced in most cell biology laboratories, as well as several cutting-edge technologies that are indeed available in a limited number of centers, but are well worth the awareness and exposure to the ECM research community. As in the previous edition, the book chapters are divided into sections that rep- sent molecular biology techniques to study gene expression, biophysical and biochemical methods for the analysis of structure and composition, cell biology methods for the asse- ment of cell behavior and matrix assembly and tissue engineering applications. All chapters were contributed by scientists who developed the methods or mastered and perfected methods that were routinely used in their laboratories. An effort was made to provide practical working details and helpful notes for the nonexpert user in order to assist reproducibility and accuracy. We hope that these valuable protocols will become helpful tools for ECM researchers and will be further developed and tailored to the s- cific needs of a growing number of applications.

Recenzje

From the reviews of the second edition: "Significant advances in the field of extracellular matrix during the past decade and current extensive investigations in this field have necessitated the second edition of this book on extracellular matrix protocols. ... well illustrated with of figures, photographs, and graphs in color and in black-and-white. ... students interested in cell biology will find this book quite useful. ... this second edition is surely welcome in the research community. Both expert and nonexpert researchers in the field of extracellular matrix will find this book extremely useful." (Omer Iqbal, Doody's Review Service, July, 2009)

Spis treści

Preface Contributors I. Molecular Biology Retroviral delivery of ECM genes Vitali Alexeev and Olga Igoucheva Tissue-Specific KO of ECM Proteins Mara Brancaccio, Emila Turco and Emilio Hirsch Recombinant Collagen Trimers from Insect Cells and Yeast- Johanna Myllyharju Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag Neil Smyth, Uwe Odenthal, Barbara Merkl, and Mats Paulsson Tissue Recombinants to Study Extracellular Matrix Targeting to Basement Membranes Patricia Simon-Assmann, Anne-Laure Bolcato-Bellemin, Annick Klein and Michèle Kedinger Preparation of recombinant fibronectin fragments for functional and structural studies David Staunton, Christopher J Millard, Radu Aricescu and Iain D Campbell II. Biochemical and biophysical analysis Quantitative Determination of Collagen Cross-links Nicholas C Avery, Trevor J Sims and Allen J Bailey ECM Macromolecules: Height-mapping and Nano-mechanics Using Atomic Force Microscopy Nigel W. Hodson, Cay M. Kielty and Michael J. Sherratt Atomic Force Microscopy Measurements of Intermolecular Binding Forces- Gradimir N. Misevic, Yannis Karamanos and Nikola J. Misevic Mass-Mapping of ECM Macromolecules by Scanning Transmission Electron Microscopy Michael J. Sherratt, Helen K. Graham, Cay M. Kielty and David F. Holmes Chemical Microscopy of Biological Samples by Dynamic Mode Secondary Ion Mass Spectrometry (SIMS) Gradimir N. Misevic, Bernard Rasser, Vic Norris, Cédric Dérue, David Gibouin, Fabrice Lefebvre, Marie-Claire Verdus, Anthony Delaune, Guillaume Legent and CamilleRipoll ECM Macromolecules: Rotary Shadowing and Transmission Electron Microscopy Michael J. Sherratt, Roger S. Meadows, Helen K. Graham, Cay M. Kielty and David F. Holmes Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Substrates Emanuele Ostuni, George M. Whitesides, Donald E. Ingber and Christopher S. Chen Solid Phase Assays for Studying ECM Protein-Protein Interactions Paul A Mould III. Cell biology assays Cell Adhesion Assays Martin J. Humphries ECM Degradation Assays for Analyzing Local Cell Invasion Vira V. Artym, Kenneth M. Yamada, Susette C. Mueller Fluorescence-Based Assays for In Vitro Analysis of Cell Adhesion and Migration Paola Spessotto, Katia Lacrima, Pier Andrea Nicolosi, Eliana Pivetta, Martina Scapolan and Roberto Perris Fibrin Gel Model for Assessment of Cellular Contractility Sharona Even-Ram Fluorescent labeling techniques for investigation of fibronectin fibrillogenesis ( Labeling fibronectin fibrillogenesis) Roumen Pankov and Albena Momchilova Stromagenesis During Tumorigenesis: Characterization of Tumor-associated Fibroblasts and Stroma-derived 3D Matrices Remedios Castelló-Cros and Edna Cukierman IV. Organ models ECM and FGF-dependent assay of embryonic SMG epithelial morphogenesis: investigating growth factor /matrix regulation of gene expression during submandibular gland development Ivan T. Rebustini and Matthew P. Hoffman Analyzing Cell-ECM Interactions in Adult Mammary Gland by Transplantation of Embryonic Mammary Tissue from Knockout Mice Teresa C. M. Klinowska and Charles H. Streuli V. Tissue

Blurb

The study of the extracellular matrix has significantly expanded over the past decade. Although thoroughly researched, the complexity and the dynamic nature of the matrix are still under extensive investigation, yielding a growing number of methods of analysis. In Extracellular Matrix Protocols: Second Edition, expert researchers explore this developing field and provide a number of techniques which can be reproduced in most cell biology labs. This edition contains methods that represent cutting edge unique technologies which are well worth exposure to the broad field and may encourage collaboration with expert labs. Chapters include information on molecular biology techniques to study gene expression, biophysical and biochemical methods for the analysis of structure and composition, and cell biology methods for assessment of cell behavior, matrix assembly and tissue engineering applications. Composed in the highly successful Methods in Molecular Biology(tm) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Extracellular Matrix Protocols: Second Edition investigates the diverse roles of the extracellular matrix, providing important tools for all researchers in the ECM community.