Experimental Neurotoxicology Methods

ISBN-13: 9781071616390 / Angielski / Miękka / 2022

Opis

This volume explores the latest methods that seek to address the vital questions being asked in neurotoxicology research. The chapters in this book cover a variety of available methods from the molecular level to the organism level, and both in vitro and in vivo approaches, including alternative model organisms. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and authoritative, Experimental Neurotoxicology Methods is a valuable resource for both young and experienced researchers who are looking for guidance to implement these methods in their laboratories or for understanding the data generated through these techniques.

Spis treści

Preface to the Series…
Preface…
Table of Contents…
Contributing Authors…

Part I Cytological and Histological Evaluation in Rodents

1. Amino-Cupric-Silver (A-Cu-Ag) Staining to Detect Neuronal Degeneration in the Mouse Brain: The de Olmos Technique
Rosario Moratalla, Adrián Sanz-Magro, and Noelia Granado

2. Assessment of Auditory Hair Cell Loss by Cytocochleograms
Aurélie Thomas, Thomas Venet, and Benoît Pouyatos

3. Evaluation of Cellular and Molecular Pathology in the Rodent Vestibular Sensory Epithelia by Immunofluorescent Staining and Confocal Microscopy
Alberto F. Maroto, Erin A. Greguske, Alejandro Barrallo-Gimeno, and Jordi Llorens

4. Morphometric Analysis of Axons and Dendrites as a Tool for Assessing Neurotoxicity
Rhianna K. Morgan, Martin Schmuck, Ana Cristina Grodzki, Donald A. Bruun, Lauren E. Matelski, and Pamela J. Lein

Part II Physiological Evaluation in Rodents

5. DPOAEs for the Assessment of Noise- or Toxicant-Induced Cochlear Damage in the Rat
Thomas Venet, Aurélie Thomas, Ludivine Wathier, and Benoît Pouyatos

6. Use of Visual Evoked Potentials to Assess Deficits in Contrast Sensitivity in Rats following Neurotoxicant Exposures
William K. Boyes

7. Electrophysiological Assessments in Peripheral Nerves and Spinal Cord in Rodent Models of Chemotherapy-Induced Painful Peripheral Neuropathy
Susanna Park, Cynthia L. Renn, Justin G. Lees, Susan G. Dorsey, Guido Cavaletti, and Valentina A. Carozzi

Part III Behavioral Evaluation in Rodents

8. The Functional Observation Battery: Utility in Safety Assessment of New Molecular Entities
David V. Gauvin

9. Behavioral Assessment of Vestibular Dysfunction in Rats
Alberto F. Maroto, Erin A. Greguske, Meritxell Deulofeu, Pere Boadas-Vaello, and Jordi Llorens

10. Assessment of Olfactory Toxicity in Dodents
David C. Dorman and Melanie L. Foster

11. Assessment of Neurotoxicant-Induced Changes in Behavior: Issues Related to Interpretation of Outcomes and Experimental Design
Deborah A. Cory-Slechta, Katherine Harvey, and Marissa Sobolewski

Part IV Molecular Methods

12. Assessment of Neurofilament Light Protein as a Serum Biomarker in Rodent Models of Toxic-Induced Peripheral Neuropathy
Giulia Fumagallia, Guido Cavalettia, Henrik Zetterberg, and Cristina Meregallia

13. Assessing Neurotoxicant-Induced Inflammation in the Central Nervous System: Cytokine mRNA with Immunostaining of Microglia Morphology
Christopher A. McPherson and G. Jean Harry

14. Mitochondrial Stress Assay and Glycolytic Rate Assay in Microglia using Agilent Seahorse Extracellular Flux Analyzers 
Gabrielle Childers and G. Jean Harry

Part V Alternative Model Organism-Based Methods

15. Assessment of Larval Zebrafish Locomotor Activity for Developmental Neurotoxicity Screening
Bridgett N. Hill, Kayla D. Coldsnow, Deborah L. Hunter, Joan M. Hedge, David Korest, Kimberly A. Jarema, and Stephanie Padilla

16. A Behavioral Test Battery to Assess Larval and Adult Zebrafish after Developmental Neurotoxic Exposure
Andrew B. Hawkey, Zade Holloway, and Edward D. Levin

17. Evaluation of Neurotoxic Effects in Zebrafish Embryos by Automatic Measurement of Early Motor Behaviors
Elisabet Teixidó, Nils Klüver, Afolarin O. Ogungbemi, Eberhard Küster, and Stefan Scholz

18. Application of Fluorescence Microscopy and Behavioral Assays to Demonstrating Neuronal Connectomes and Neurotransmitter Systems in C. elegans
Omamuyovwi M. Ijomone, Priscila Gubert, Comfort O. A. Okoh, Alexandre M. Varão, Leandro de O. Amara, Oritoke M. Aluko, and Michael Aschner

Part VI In Vitro Methods

19. Effects of Neurotoxic or Pro-Regenerative Agents on Motor and Sensory Neurite Outgrowth in Spinal Cord Organotypic Slices and DRG Explants in Culture
Sara Bolivar, Ilary Allodi, Mireia Herrando, and Esther Udina

20. Integrative In Vitro/Ex Vivo Assessment of Dopaminergic Neurotoxicity in Rodents using Striatal Synaptosomes and Membrane Preparations
Raúl López-Arnau and David Pubill

21. Quantification of Oligodendrocytes and Myelin in Human iPSC-Derived 3D Brain Cell Cultures (Brainspheres)
David Pamies, Megan Chesnut, Hélène Paschoud, Marie-Gabrielle Zurich, Thomas Hartung, and Helena T. Hogberg

22. Measurement of Electrical Activity of Differentiated Human iPSC-Derived Neurospheres Recorded by Microelectrode Arrays (MEA)
Kristina Bartmann, Julia Hartmann, Julia Kapr, and Ellen Fritsche

Subject Index List…