Evaluation of the anti-GFP tandem nano body LaG-16- G4S-LaG2 : and its applicability in protein purification

ISBN-13: 9786202215008 / Angielski / Miękka / 2018 / 68 str.

Georgi Nikolov

Opis

Nanobodies are an unconventional type of antibodies. Also known as heavy-chain antibodies, they are found in the sera of Camelidae and are devoid of the L-chain polypeptide, while also lacking the first constant domain - CH1. These properties, as well as their relatively smaller size allow them to be well adapted for accessing binding sites of difficult targets. A prime example of a versatile nanobody is the tandem nanobody LaG-16-G4S-LaG-2, developed by the Rout lab, as this group has shown its high specificity and affinity for GFP. The strong affinity of the tandem nanobody for this marker molecule would allow its utilization in the purification of low-abundance proteins or protein complexes. Here I present experiments aimed at rendering the LaG-16-G4S-LaG-2-GFP protein capture system and its subsequent combination with the FimGt-DsF binding complex accessible for future structural studies. Furthermore, I show how the system can be improved upon in the future. I am certain that the experiments at hand will help to illuminate the crucial steps of the novel protein purification approach and can lead t

O autorze

Nikolov, Georgi
Georgi Nikolov is an aspiring scientist who has worked in the fields of bioinformatics, structural biology and most recently, microbial ecology. Having completed his studies in both Glasgow and Vienna, he now seeks further scientific knowledge as a PhD student in the University of Vienna.