Evaluation of antiviral and cytotoxic activity

ISBN-13: 9783846521922 / Angielski / Miękka / 108 str.

Waqas Ahmad; Muhammad Ashraf; Aqeel Javeed

Opis

The antiviral activity of plants Glyceriza glabra (roots), Phyllanthus amblicus (Fruit), Eugenia jamolana (Leaves), and Moringa oleifera (Leaves) were evaluated against Infectious bursal disease virus (IBDv) in this study. Ethanolic extraction of these plants was carried out by using Soxhlet apparatus. Four dilutions of each extracts viz 100, 50, 25 and 12.5 g/ml were made. Vero cells were infected by identified infectious bursal disease for antiviral assay. Dilutions of these extracts were applied in triplicate manner on Vero cells (infected by IBDv), that are confluent in 96 well cell culture plates. The cytotoxic activity of mentioned plant extracts was carried out by treating the cells by the same dilutions as used in antiviral assay and incubated the both 96 well cell culture plates for four days. After this incubation, viability of cells was determined by MTT [3-(4,5-dimethylthiazol 2-yl)-2,5-diphenyltetrazolium bromide] colorimetric assay. Endpoint of these assays was measured in terms of cell survival percentage. Results were compared for qualitative variables using Chi-square technique and quantitative variables by linear regression analysis.

O autorze

Dr. Waqas Ahmad completed Pharm.D from University college of Pharmacy, University of the Punjab, Lahore in 2008. He completed his M.Phil in discipline of Pharmacology and Toxicology from University of Veterinary and Animal Sciences, Lahore in 2011. He is currently working as Lecturer at Department of Pharmacy, University of Lahore, Pakistan.