Part 1. Basic information about HPLC1. Introductory information regarding HPLC 2. Overview of HPLC instrumentation and its use3. Parameters for the characterization of HPLC separation4. Equilibrium types in HPLC5. Intermolecular forces6. Characterization of analytes and matrices 7. Mobile phases and their properties8. Analytical HPLC columns and their characteristics
Part 2. Main types of HPLC separations 9. Reversed-phase HPLC10. Other HPLC separations performed on hydrophobic stationary phases11. Hydrophilic interaction chromatography (HILIC)12. Other HPLC separations performed on polar stationary phases13. Ion-exchange, ion-moderated, and ligand-exchange liquid chromatography14. Chiral HPLC Separations15. Size exclusion HPLC16. Affinity, immunoaffinity, and aptamer type HPLC17. Mixed mode HPLC
Part 3. Practice of HPLC analysis18. Utilization of HPLC in chemical analysis
Part 4. Appendices
Dr. Serban C. Moldoveanu is Senior Principal Scientist at R. J. Reynolds Tobacco Company. His research activity is focused on various aspects of chromatography including method development for the analysis by GC/MS, HPLC, and LC/MS/MS of natural products and cigarette smoke. He has also performed research on pyrolysis of a variety of polymers and small molecules. He has over 100 publications in peer reviewed journals, eleven books, and several chapter contributions. He is a member of the editorial board of the Journal of Analytical Methods in Chemistry.
Dr. Victor David is a Professor and Head of the Department of Analytical Chemistry, University of Bucharest, Romania. He is the author of more than 120 publications, including 92 scientific papers in ISI international journals and 10 books and chapters in various Encyclopedias. He is also a reviewer at several international journals.