Erbb Receptor Signaling: Methods and Protocols

ISBN-13: 9781493972180 / Angielski / Twarda / 2017 / 303 str.

Zhixiang Wang

Opis

This volume contains protocols specifically designed for studying cell signaling mediated by ErbB receptors, all applicable to the study of a broad range of ErbB receptor-mediated signaling from basic research to clinic applications, from cultured cells to various animal models and primary cancer cells from patients.

Spis treści

Part I: Introduction and Review

 

1. ErbB Receptors and Cancer

            Zhixiang Wang

 

2. New Insights from Drosophila into the Regulation of EGFR Signaling

            Nicholas Harden

 

3. C. elegans Vulva Induction: An In Vivo Model to Study Epidermal Growth Factor Receptor Signaling and Trafficking

            Kimberley Gauthier and Christian E. Rocheleau

 

4. Targeting HER2 in Advanced Breast Cancer

            Xiaofu Zhu and Anil Abraham Joy

 

Part II: Classical Methods in the Studies of ErbB Receptor Signaling

 

5. Methods to Investigate EGFR U

biquitination

            Alexia Conte and Sara Sigismund

 

6. Dimerization Assessment of Epithelial Growth Factor Family of Receptor Tyrosine Kinases by Using Cross-Linking Reagent

            Hamid Maadi, Babak Nami, and Zhixiang Wang

 

7. Application of Immunofluorescence Staining to Study ErbB Family of Receptor Tyrosine Kinases

            Babak N. Mollalou and Zhixiang Wang

 

8. Activation of Endosome-Associated Inert EGF Receptor Following Internalization

            Yi Wang, Sukhmani Billing, and Zhixiang Wang

 

9. Two-Pulse Endosomal Stimulation of Receptor Tyrosine Kinases Induces Cell Proliferation

 &

nbsp;          Steven Pennock, Sukhmani Billing, Zhixiang Wang, and Yi Wang

 

10. Study of EGFR Signalling/Endocytosis by Site-Directed Mutagenesis

            Qian Wang and Zhixiang Wang

 

11. Using Percoll Gradient Fractionation to Study the Endocytic Trafficking of the EGFR

            Julie S. Gosney and Brian P. Ceresa

 

12. Analysis of Epidermal Growth Factor Receptor-Induced Cell Motility by Wound Healing Assay

            Junfeng Tong and Zhixiang Wang

 

Part III: Recently Developed Methods in the Studies of ErbB Receptor Signaling

 

13. Cell Cycle Synchronization of HeLa Cells to Assay EGFR Pathway A

ctivation

            Ping Wee and Zhixiang Wang

 

14. Analysis of Constitutive EGFR Signaling Regulating IRF3 Transcriptional Activity in Cancer Cells

            Gao Guo, Ke Gong, and Amyn A. Habib

 

15. Measurement of Epidermal Growth Factor Receptor-Derived Signals Within Plasma Membrane Clathrin Structures

            Stefanie Lucarelli, Ralph Christian Delos Santos, and Costin N. Antonescu

 

Part IV: Methods for Studying EGFR Signaling in Drosophila

 

16. Studying Non-Proliferative Roles for Egfr Signaling in Tissue Morphogenesis Using Dorsal Closure of the Drosophila Embryo

            Bruce Reed and Nicholas H

arden

 

Part V: Methods Related to the Translational Research of ErbB Receptor Signaling

 

17. Analysis of Epithelial-Mesenchymal Transition Induced by Overexpression of Twist

            Jing-Wen Bai, Yong-Qu Zhang, Yao-Chen Li, and Guo-Jun Zhang

 

18. Assessment of Specificity of an Adenovirus Targeted to HER3/4

            Sheena H. MacLeod, Kyle G. Potts, Shyambabu Chaurasiya, and Mary M. Hitt

 

19. Isolation of Human Mesenchymal Stem Cells for Studying ErbB Receptor Signaling

            Chao Chen and Hongxing Jiang

Blurb

This volume contains protocols specifically designed for studying cell signaling mediated by ErbB receptors, all applicable to the study of a broad range of ErbB receptor-mediated signaling from basic research to clinic applications, from cultured cells to various animal models and primary cancer cells from patients. After some overviews, the book continues by covering common protocols for studying various aspects of ErbB receptor-mediated cell signaling, newly-developed methods in biomedical research that are also widely-used in the study of ErbB receptor signaling, epidermal growth factor receptor (EGFR) signaling in Drosophila, as well as protocols for studying ErbB receptor signaling in various animal model systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Detailed and easy-to-follow, ErbB Receptor Signaling: Methods and Protocols serves as an ideal guide for researchers who are studying the cell signaling mediated by ErbB receptors and other receptor tyrosine kinases.