Epstein Barr Virus: Methods and Protocols

ISBN-13: 9781493966530 / Angielski / Twarda / 2016 / 269 str.

Janos Minarovits; Johann Helmut Niller

Opis

This volume explores data from the applications of molecular biological methods and the applications of recent immunological and cytogenetic methods in Epstein-Barr Virus (EBV) that will offer readers possible new solutions to the unresolved problems in the EBV field.

Recenzje

"This is an excellent book with a good number of color illustrations providing recent immunological and cytogenetic methods and protocols aimed at finding easy solutions to unresolved problems in the field of Epstein Barr virus. ... The book is appropriate for students and scientists interested in Epstein Barr Virus (EBV) and EBV diagnostics. ... The book has high quality color illustrations to promote easy understanding of this complex subject." (Omer Iqbal, Doody's Book Reviews, April, 2017)

Spis treści

Foreword…
Preface…
Table of Contents…
Contributing Authors…

 

1. Current Trends and Alternative Scenarios in EBV Research
Janos Minarovits and Hans-Helmut Niller

2. Epstein-Barr Virus: Clinical Diagnostics
Hans-Helmut Niller and Georg Bauer

3. Establishment of EBV-Infected Lymphoblastoid Cell Lines
Noemi Nagy

4. Generation and Infection of Organotypic Cultures with Epstein-Barr Virus
Rachel M. Temple, Craig Meyers, and Clare E. Sample

5. Affinity Purification-Mass Spectroscopy Methods for Identifying Epstein-Barr Virus-Host Interactions
Anna A. Georges and Lori Frappier

6. The Use of 3D Telomere FISH for the Characterization of the Nuclear Architecture in EBV-Positive Hodgkin’s Lymphoma

   Hans Knecht and Sabine Mai

7. Analysis of EBV Transcription Using High-Throughput RNA Sequencing

  Tina O’Grady, Melody Baddoo, and Erik K. Flemington

8. Analysis of Viral Promoter Usage in EBV-Infected Cell Lines: A Comparison of qPCR following Conventional RNA Isolation and Nuclear Run-On Assay

  Kalman Szenthe and Ferenc Banati

9. Analysis of Viral and Cellular MicroRNAs in EBV Infected Cells

   Rebecca L. Skalsky

10. Isolation and Characterization of Exosomes Released by EBV Immortalized Cells
Gulfaraz Khan and Waqar Ahmed

11. Functional Analysis of Exosomes Derived from EBV-Infected Cells
Gulfaraz Khan and Pretty S. Philip

12. Terminal Repeat Analysis of EBV Genomes
   Ferenc Bánáti, Anita Koroknai, Kálmán Szenthe

13. Characterization of EBV Promoters and Coding Regions by Sequencing PCR-Amplified DNA Fragments
Kalman Szenthe and Ferenc Bánáti

14. The Use of Chromatin Precipitation Coupled to DNA Sequencing (ChIP-Seq) for the Analysis of Zta Binding to the Human and EBV Genome
Anja Godfrey, Sharada Ramasubramanyan, and Alison J. Sinclair

15. Analysis of Viral Epigenotypes Using Bisulfite Sequencing: A Detailed Protocol for the Crucial Bisulfite Modification and PCR Amplification Steps

      Daniel Salamon

<16. Analysis of Viral Epigenotypes using Chromatin Immunoprecipitation

   Ferenc Bánáti and Kálmán Szenthe

17. Mice with Reconstituted Human Immune System Components as a Tool to Study Immune Cell Interactions in EBV Infection

       Frank Heuts and Noemi Nagy

18. Generation and Analysis of Humanized Mouse Model of EBV Infection

     Ken-Ichi Imadome and Shigeyoshi Fujiwara

19. EBV-Directed T Cell Therapeutics for EBV-Associated Lymphomas

     Lauren P. McLaughlin, Stephen Gottschalk, Cliona M. Rooney, and Catherine M. Bollard 

Blurb

This volume explores data from the applications of molecular biological methods and the applications of recent immunological and cytogenetic methods in Epstein-Barr Virus (EBV) that will offer readers possible new solutions to the unresolved problems in the EBV field. Chapters in this book cover topics such as: viral life cycle, latency, EBV-associated diseases and EBV diagnostics; in vitro methods including organotypic cultures for the analysis of EBV-epithelial cell interactions; identification of the interacting viral and cellular proteins using affinity purification-mass spectrometry methods; 3D telomere FISH; transcription analysis using high-throughput RNA sequencing, qPCR and nuclear run-on assay; analysis of viral and cellular microRNAs; isolation and characterization of exosomes and the assessment of their function; characterization of the viral genome by terminal repeat analysis and sequencing; the use of chromatin immunoprecipitation coupled sequencing (ChIP-Seq) for the analysis of Zta-DNA interactions; epigenetic analysis by bisulfite sequencing and ChIP; novel in vivo models for the study of EBV infection; and how immunological, virological, tissue culture and molecular methods can be combined to yield Good Manufacturing Practice-compliant EBV-specific T cells for the immunotherapy of EBV-associated post-transplant lymphoproliferative diseases (PTLD). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Epstein Barr-Virus: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field.