Epithelial Cell Culture Protocols

ISBN-13: 9781627031240 / Angielski / Twarda / 2012 / 469 str.

Scott H. Randell; M. Leslie Fulcher

Opis

Fascinating biology occurs at epithelial interfaces, whether between organism and environment or within body compartments, and many diseases inflicting huge personal and societal burdens result from dysfunction of epithelial systems, e.g., carcinomas. Epithelial cell cultures have been an integral and crucial part of the biomedical research enterprise, adding unique capabilities and enabling mechanistic approaches. In the past decade there have been many research advances, such as directed differentiation of embryonic stem cells and induced pluripotent stem cells, robotic high throughput screening, whole genome siRNA and shRNA libraries, massively parallel sequencing at low cost, identification of somatic stem cells in key organs, to name a few. Epithelial Cell Culture Protocols, Second Edition provides a cross section of up-to-date culture protocols for the most heavily studied cell systems and featured supporting technologies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Epithelial Cell Culture Protocols, Second Edition will serve outstanding investigators with the best possible information for the advancement of biomedical science.

Spis treści

1. Progress Towards Drosophila Epithelial Cell Culture

            Amanda Simcox

 

2. Culture of Choriod Plexus Epithelial Cells and In Vitro Model of Blood-CSF Barrier

            Andrew D. Monnot and Wei Zheng           

 

3. Methods for Culture of Human Corneal and Conjunctival Epithelia

            Sandra J. Spurr-Michaud and Ilene K. Gipson

 

4. The Culture and Maintenance of Functional Retinal Pigment Epithelial Monolayers from Adult Human Eye

            Timothy A. Blenkinsop, Enrique Salero, Jeffrey Stern, and Sally Temple

 

5. Oral and Pharyngeal Epithelial Keratinocyte Culture

            Kantima Leelahavanichkul and J. Silvio Gutkind   

 

6. Primary Culture of the Human Olfactory Neuroepithelium

            Nancy E. Rawson and Mehmet Hakan Ozdener

 

7. Primary Culture of Mammalian Taste Epithelium

            Mehmet Hakan Ozdener and Nancy E. Rawson    

 

8. Human Nasal and Tracheo-Bronchial Respiratory Epithelial Cell Culture

            M. Leslie Fulcher and Scott H. Randell      

 

9. Culture and Differentiation of Mouse Tracheal Epithelial Cells

            Yingjian You and Steven L. Brody  

 

10. Isolation and Culture of Alveolar Epithelial Type I and Type II cells from Rat Lungs

            Robert F. Gonzalez and Leland G. Dobbs

 

11. Classical Human Epidermal Keratinocyte Cell Culture

            Cathy Rasmussen, Christina Thomas-Virnig, and B. Lynn Allen-Hoffmann      

 

12. Mouse Epidermal Keratinocyte Culture

            Luowei Li

 

13. Three Dimensional Culture of Human Breast Epithelial Cells: The How and The Why

            Pierre-Alexandre Vidi, Mina J. Bissell, and Sophie A. Lelièvre

 

14. Three Dimensional Cultures of Mouse Mammary Epithelial Cells

            Rana Mroue and Mina J. Bissell      

 

15. Isolation, Characterisation and Re-aggregate Culture of Thymic Epithelial Cells

            Natalie Seach, Maree Hammett, and Ann Chidgey              

 

16. Enrichment and Clonal Culture of Hepatic Stem/Progenitor Cells During Mouse Liver Development

            Akihide Kamiya and Hiromitsu Nakauchi

 

17. Long Term Culture and Co-Culture of Primary Rat and Human Hepatocytes

            Maria Shulman and Yaakov Nahmias        

 

18. Tissue-Culture Correlational Study of Genetic Cholangiopathy of Autosomal Recessive Polycystic Kidney Disease

            Yasuni Nakanuma, Yasunoi Sato, and Kenichi Harada    

 

19. Primary Mouse Small Intestinal Epithelia Cell Cultures

            Toshiro Sato and Hans Clevers                 

 

20. Isolation, Growth and Characterization of Human Renal Epithelial Cells Using Traditional and 3D Methods

            J.J. Gildea, H.E. McGrath, R. E. VanSciver, Wang D. Bigler, and Robin A. Felder 

 

21. Human Female Reproductive Tract Epithelial Cell Culture

            Rachel W. S. Chan, Abby S. C. Mak, William S. B. Yeung, Kai-Fai Lee, Annie N. Y. Cheung, Hextan Y. S. Ngan, and Alice S. T. Wong

 

22. Primary Culture and Propagation of Human Prostate Epithelial Cells

Birunthi Niranjan, Mitchell G. Lawrence, Melissa M. Papargiris, Michelle G. Richards, Shirin Hussain, Mark Frydenberg, John Pedersen, Australian Prostate Cancer BioResource, Renea A. Taylor, and Gail P. Risbridger

             

23. Urothelial Cell Culture: Stratified Urothelial Sheet and Three-Dimensional Growth of Urothelial Structures

            Yuanyuan Zhang and Anthony Atala                                

 

24. Analysis of Tissue Interactions in Ectodermal Organ Culture

            Pauliina M. Munne, Katja Närhi, and Frederic Michon

 

25. Generation of Human Cell Lines Using Lentiviral-Mediated Genetic Engineering

            Patrick Salmon                    

 

26. Viral Transformation of Epithelial Cells

            Jennifer A. Regan and Laimonis A. Laimins