Epidermal Cells: Methods and Protocols

ISBN-13: 9781071602539 / Angielski / Miękka / 2020 / 301 str.

Kursad Turksen

Spis treści

1. The Simplest Protocol for Rapid and Long-Term Culture of Primary Epidermal Keratinocytes from Human and Mouse

            Filipa Pinto, Daisuke Suzuki, and Makoto Senoo

 

2. Dermal-Epidermal Separation by Heat

            Liyan Jian, Yu Cao, and Ying Zou

 

3. Dermal-Epidermal Separation by Enzyme

            Liyan Jian, Yu Cao, and Ying Zou

 

4. Dermal-Epidermal Separation by Chemical

            Liyan Jian, Yu Cao, and Ying Zou

 

5. Isolation and Characterization of Extracellular Vesicles from Keratinocyte Cultures

            Sebastian Sjöqvist, Aya Imafuku, Dhanu Ghupta, and Samir EL Andaloussi

 

6. Competitive Repopulation Assay of Long-Term Epidermal Stem Cell Regeneration Potential

            Carmen Segrelles, Karla Santos-de-Frutos, Jesús M. Paramio, and Corina Lorz

 

7. Quantification of Melanosome Transfer Using Immunofluorescence Microscopy and Automated Image Analysis

            Aishwarya Sridharan, John Lim S.Y., Graham D. Wright, and Leah A. Vardy

 

8. Contribution of Immunohistochemistry in Revealing S100A7/JAB1 Colocalization in Psoriatic Epidermal Keratinocyte

            Mariagrazia Granata, Evangelia Skarmoutsou, Maria Clorinda Mazzarino, Massimo Libra, and Fabio D’Amico

 

9. Digital Quantification of Epidermal Protein Expression in Paraffin-Embedded Tissue Using Immunohistochemistry

            Manuel Valdebran, Eric H. Kowalski, Diana Kneiber, Jing Li, Jeffrey Kim, and Kyle T. Amber

 

10. Keratinocyte Differentiation by Flow Cytometry

            Natalia Sanz-Gómez, Ana Freije, and Alberto Gandarillas

 

11. In Silico and In Vitro Considerations of Keratinocyte Nuclear Receptor Protein Structural Order for Improving Experimental Analysis

            Rambon Shamilov, Matthew J. Staid, and Brian J. Aneskievich

 

12. Genetic Modification of Human Primary Keratinoctes by Lentiviral Vectors

            Ana Freije, Natalia Sanz-Gómez, and Alberto Gandarillas

 

13. Generation of Knockout Human Primary Keratinocytes by CRISPR/Cas9

            Serena Grossi, Gabriele Fenini, Paulina Hennig, Michela Di Filippo, and Hans-Dietmar Beer

 

14. In Vitro Wound Healing Assays to Investigate Epidermal Migration

            Kegan Main, Constantinos M. Mikelis, and Colleen L. Doçi

 

15. Iterative Three-Dimensional Epidermis Bioengineering and Xenografting to Assess Long-Term Regenerative Potential in Human Keratinocyte Precursor Cells

            Nicolas O. Fortunel, Emmanuelle Bouissou-Cadio, Julien Coutier, and Michèle T. Martin

 

16. Generation of a Full-Thickness Human Skin Equivalent on an Immunodeficient Mouse

            Nicole Diette, Igor Kogut, and Ganna Bilousova

 

17. Biotin Identification Proteomics in Three-Dimensional Organotypic Human Skin Cultures

            Calvin J. Cable, Nihal Kaplan, Spiro Getsios, Paul M. Thomas, and Bethany E. Perez White

 

18. Information and Statistical Analysis Pipeline for High-Throughput RNA Sequencing Data

            Shinji Nakaoka and Keita Matsuyama

 

19. Cell-Extracellular Matrix Adhesion Assay

            Mehmet Varol

 

20. Methods for Analysis of Keratinocyte Migration

            Jie Liu and Jiaping Zhang

 

21. Scratch Wound Healing Assay

            Simona Martinotti and Elia Ranzato

 

22. Photoprotective Activity Assay toward Ultraviolet B in Human Keratinocytes

            Mehmet Varol

 

23. Photodynamic Therapy Assay

            Mehmet Varol

 

24. A Method to Prepare Claudin-Modulating Recombinant Proteins

            Keisuke Tachibana and Masuo Kondoh

 

25. Human Fetal Skin Fibroblast Isolation and Expansion for Clinical Application

            Parisa Goodarzi, Hamidreza Aghayan, Moloud Payab, Bagher Larijani, Sepideh Alavi-Moghadam, Masoumeh Sarvari, Hossein Adibi, Fatemeh Khatami, Najmeh Foroughi Heravani, Mahdieh Hadavandkhani, and Babak Arjmand

 

26. Ciliated Epithelial Cell Differentiation at Air-Liquid Interface Using Commercially Available Culture Media

            Dani Do Hyang Lee, Alina Petris, Robert E. Hynds, and Christopher O’Callaghan

Blurb

This fully updated edition brings together a new set of protocols to arm epidermal cell biologists with tools and approaches to continue the quest to uncover the intricacies and regulatory mechanisms underlying epidermal cell development and function. The collection takes a detailed view at techniques currently being applied to this developing field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and cutting-edge, Epidermal Cells: Methods and Protocols, Fourth Edition serves as a valuable reference for experts as well as novices in the epidermal cell field.