Eosinophils: Methods and Protocols

ISBN-13: 9781493955091 / Angielski / Miękka / 2016 / 324 str.

Garry M. Walsh

Opis

In Eosinophil: Methods and Protocols, experts in the field of eosinophil biology comprehensively provide detailed methodological insight into the study of this fascinating cell. This book is aimed at a diverse range of basic and clinical scientists who wish to work with eosinophils or who require an update of their knowledge or to gain the information required to study a function of the eosinophil different to their current area of enquiry.​ Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Eosinophil: Methods and Protocols seeks to aid scientist in the discovery of new hypotheses and for further examination of this intriguing cell.

Spis treści

1.                  Eosinophil Overview  -  Structure, Biological Properties And Key Functions
Paige Lacy, Helene F. Rosenberg, Garry M. Walsh

2.                  Eosinophil Purification From Peripheral Blood
Praveen Akuthota, Kelsey Capron And Peter F. Weller

3.                  Eosinophil Purification From Human Bone Marrow
Tina W. Wong And Diane F. Jelinek

4.                  CD34(+) Eosinophil-Lineage-Committed Cells In The Mouse Lung
Apostolos Bossios And Madeleine Rådinger

5.                  Eosinophil Cell Lines
Kenji Ishihara

6.                  Cell Signalling In Eosinophil During Human Eosinophil Differentiation
Miranda Buitenhuis

7.                  Eosinophil Intracellular Signalling – Apoptosis
 Pinja Ilmarinen, Eeva Moilanen And Hannu Kankaanranta

8.                  Identification Of Human Eosinophils In Whole Blood By Flow Cytometry
Caroline Ethier, Paige Lacy And Francis Davoine

9.                  Isolation And Functional Assessment Of Eosinophil Crystalloid Granules
Renata Baptista-Dos-Reis, Valdirene S. Muniz And Josiane S. Neves

10.              Eosinophil Chemotaxis
Gordon Dent

11.              Human Eosinophil Shape Change And Secretion
Lian Willetts And Paige Lacy

12.              Human Eosinophil Adhesion And Receptor Expression
Colleen S. Curran

13.              Adhesion Of Eosinophils To Endothelial Cells Or Substrates Under Flow Conditions
Viktoria Konya,  Miriam Peinhaupt And Akos Heinemann

14.              Human Eosinophil Transmigration
Stanislawa Bazan-Socha, Joanna Zuk, Bogdan Jakiela And Jacek Musial

15.              Measurement Of Eosinophil Kinetics In Healthy Volunteers
Neda Farahi, Chrystalla Loutsios, Rosalind P. Simmonds, Linsey Porter, Daniel Gillett, Sarah Heard, A. Michael Peters, Alison M. Condliffe And Edwin R. Chilvers

16.              Assays Of Eosinophil Apoptosis And Phagocytic Uptake
David A. Dorward, Sidharth Sharma, Ana L. Alessandri, Adriano G. Rossi, Christopher D. Lucas

17.              Clinical Measurement Of Eosinophil Numbers In Eosinophilic Conjunctivitis
Osmo Kari And K. Matti Saari

18.              Qualitative And Quantitative Studies Of Eosinophils In Parasitic Infections
Masataka Korenaga And Fabrizio Bruschi

19.              Interactions Of Eosinophils With Nerves
Quinn R. Roth-Carter, David B. Jacoby And Zhenying Nie

20.              Eosinophils Interaction With Mast Cells: The Allergic Effector Unit
Roopesh Singh Gangwar And Francesca Levi-Schaffer

21.              Eosinophil Interactions: Antigen Presentation
Praveen Akuthota

22.              Eosinophils And Respiratory Virus Infection: A Dual-Standard Curve Qrt-PCR-Based Method For Determining Virus Recovery From Mouse Lung Tissue
Caroline M. Percopo, Kimberly D. Dyer, Kendal A. Karpe, Joseph B. Domachowske And Helene F. Rosenberg

23.              Antimicrobial Activity Of Human Eosinophil Granule Proteins
Anu Chopra, And Janendra K. Batra

24.              Eosinophils And The Ovalbumin Mouse Model Of Asthma
F. Daubeuf And N. Frossard

25.              Mutant Mice And Animal Models Of Allergic Disease
Marie-Renée Blanchet, Matthew Gold And Kelly M Mcnagny

26.              Murine Models Of Eosinophilic Leukemia - A Model Of FIP1L1-PDGFRa  Initiated Chronic Eosinophilic Leukemia/Systemic Mastocytosis
Yoshiyuki Yamada, Jose A. Cancelas And Marc E. Rothenberg

Blurb

In Eosinophils: Methods and Protocols, experts in the field of eosinophil biology comprehensively provide detailed methodological insight into the study of this fascinating cell. This book is aimed at a diverse range of basic and clinical scientists who wish to work with eosinophils or who require an update of their knowledge or to gain the information required to study a function of the eosinophil different to their current area of enquiry.​Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Eosinophils: Methods and Protocols seeks to aid scientist in the discovery of new hypotheses and  for further examination of this intriguing cell.