Enzymology at the Membrane Interface: Interfacial Enzymology and Protein-Membrane Binding: Volume 583

ISBN-13: 9780128094198 / Angielski / Twarda / 2017 / 430 str.

Michael H. Gelb

Opis

Enzymology at the Membrane Interface, the latest volume in the Methods in Enzymology series, covers a subset of enzymes that work in the environment of the biological cell membrane. This field, called interfacial enzymology, involves a special series of experimental approaches for the isolation and study of these enzymes.

• Covers a subset of enzymes that work in the environment of the biological cell membrane
• Offers a series of experimental approaches for the isolation and study of enzymes

Spis treści

1. A High-Throughput Flurometric Assay for Lipid-Protein Binding Wonhwa Cho 2. Fluorescence-based in situ quantitative imaging for cellular lipids Wonhwa Cho 3. Recombinant Expression of human and mouse groups I, II, III, V, V and XII Secreted Phospholipases A2 Michael H. Gelb and Gerard Lambeau 4. Vesicle-based assay of secreted phospholipase A2 enzymatic activity for high throughput screening of compound libraries Michael H. Gelb 5. Cellular Assays for Evaluating Calcium-Dependent Translocation of cPLA2a to Membrane Christina C. Leslie 6. Secreted Phospholipase A2 Specificity on Natural Membrane Phospholipids Makoto Murakami 7. Analyses of Calcium-Independent Phospholipase A2beta (iPLA2ß) in Biological Systems Sasanka Ramanadham 8. Using hydrogen deuterium exchange mass spectrometry to examine protein membrane interactions John E. Burke 9. Lipid-binding C2 domains mediate a diverse class of enzymatic functions" Robert V. Stahelin 10. Interfacial Enzymes: Membrane Binding, Orientation, Membrane Insertion, and Activity Suren Tatulian 11. Enzymology at the Membrane Interface Dan M. Raben 12. Studying gastric lipase adsorption onto phospholipid monolayers by surface tensiometry, ellipsometry and atomic force microscopy Frédéric Carrière 13. Probing conformational changes and interfacial recognition site of lipases with surfactants and inhibitors Frédéric Carrière 14. Measuring Phospholipase D enzymatic activity through biochemical and imaging methods Michael A. Frohman 15. Pld Rotein-Protein Interaction with Intracellular Signaling Molecules, Modulation by Mitogen Pa and Pld's Gef Activity Julian G. Cambronero 16. Phosphatidic Acid Regulation of PIPKI Guangwei Du

O autorze

Michael H. Gelb studied chemistry and biochemistry as an undergraduate at the University of California at Davis. His Ph.D. studies with Stephen G. Sligar at Yale University led to a better understanding of the catalytic mechanism of cytochrome P450. As an American Cancer Society Postdoctoral Fellow in the laboratory of the late Robert H. Abeles at Brandeis University, Gelb studied a variety of mechanism-based inactivators of serine proteases and developed fluorinated ketones as tight-binding inhibitors of several classes of proteases. In 1985 Gelb became a faculty member in the Departments of Chemistry and Biochemistry at the University of Washington. Major breakthroughs in the group include the development of methods to properly analyze the action of enzymes on membrane surfaces, the discovery of protein prenylation (farnesylation and geranylgeranylation) in mammalian cells (together with John A. Glomset), the development of Isotope-Coded Affinity Tags (ICAT reagents) for proteomic applications (together with Ruedi Aebersold), and the development of newborn screening for lysosomal storage diseases by mass spectrometry.