1. Detection of Caenorhabditis elegans Germ Cell Apoptosis Following Exposure to Environmental Contaminant Mixtures: A Crude Oil-Dispersant Mixture Example
Joseph Ryan Polli, Mary Farwell, and Xiaoping Pan
2. High-Throughput Measurement for Toxic Effects of Metal Mixtures in Caenorhabditis elegans
Kathy S. Xue and Lili Tang
3. Evaluations of Environmental Pollutants-Induced Mitochondrial Toxicity Using Caenorhabditis elegans as a Model System
Fuli Zheng, Michael Aschner, and Huangyuan Li
4. Methods to Assay the Behavior of Drosophila melanogaster for Toxicity Study
Guiran Xiao
5. Investigating the Joint Effects of Pesticides and Ultraviolet-B Radiation in Xenopus laevis and Other Amphibians
Shuangying Yu and Mike Wages
6. Identification of Stable Reference Genes for Toxicogenomic and Gene Expression Analysis
Xiaoping Pan and Baohong Zhang
7. Semi-Quantitative RT-PCR: An Effective Method to Explore the Regulation of Gene Transcription Level Affected by Environmental Pollutants
Fan Wang
8. Employing Multiple New Neurobiological Methods to Investigate Environmental Neurotoxicology in Mice
Ping Cai and Huangyuan Li
9. Analyses of Epigenetic Modification in Environmental Pollutants-Induced Neurotoxicity
Guangxia Yu, Zhenkun Guo, and Huangyuan Li
10. The Application of Omics Technologies in the Research of Neurotoxicology
Wenya Shao and Huangyuan Li
11. Flow Cytofluorometric Analysis of Molecular Mechanisms of Premature Red Blood Cell Death
Mohammad A. Alfhili and Myon Hee Lee
12. Practical Methods and Technologies in Environmental Epidemiology
Chuancheng Wu, Donghong Wei, Huangyuan Li, and Siying Wu
13. In Ovo Early-in-Life Inhalation Exposure to Gas/Aerosol with a Chicken Embryo Model
14. DNA Damage in Liver Cells of the Tilapia Fish Oreochromis mossambicus Larva Induced by the Insecticide Cyantraniliprole at Sublethal Doses During Chronic Exposure
Yongmei Fan, Chengbin Xu, and Weiguo Miao
Part II: Plant Toxicity
15. Impact of Nanoparticles on Plant Growth, Development, and Biomass
Ilya N. Boykov and Baohong Zhang
16. Biochemical and Physiological Toxicity of Nanoparticles in Plant
Zhiyong Zhang and Baohong Zhang
17. Determination of Oxidative Stress and Antioxidant Enzyme Activity for Physiological Phenotyping During Heavy Metal Exposure
Samrana Zahir, Fan Zhang, Jinhong Chen, and Shuijin Zhu
18. Comprehensive Phytotoxicity Assessment Protocol for Engineered Nanomaterials
Lok R. Pokhrel, Chukwudi S. Ubah, and Sina Fallah
19. Detection of Cadmium Toxicity in Plant
Xiaoxiao Liu, Lina Yin, Shiwen Wang, and Zhiyong Zhang
Part III: Microbe Toxicity
20. Mutagenicity Evaluation of Nanoparticles by the Ames Assay
Xiaoping Pan
Part IV: Extraction and Detection Analytical Methods
21. Dispersive Solid Phase Extraction of Multiresidue Pesticides in Food and Water Samples
Hongxia Guan
22. Quantitative Analysis of Multiresidue Pesticides Using Gas Chromatography/Mass Spectrometry
Qingsong Cai and Hongxia Guan
23. Determination of the N-Nitroso Compounds in Mouse Following RDX Exposure
Xiaoping Pan
24. Determination of Metal Content in Drosophila melanogaster During Metal Exposure
Guiran Xiao
25. Microplastics: A Review of Methodology for Sampling and Characterizing Environmental and Biological Samples
Christiana H. Shoopman and Xiaoping Pan
This detailed book provides an accessible compendium of up-to-date methods in the fields of environmental toxicology, molecular toxicology, and toxicogenomics. Organized into four major sections, the volume examines methods utilizing model animal species, such as nematode, fruit fly, mice, chicken, and amphibians, methods using plants to study chemical toxicity, applying the Ames assay to chemical mutagenicity study, as well as methods for environmental chemical analysis. Although this book is divided into these parts, the methods can be used across species. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Environmental Toxicology and Toxicogenomics: Principles, Methods, and Applications serves as a valuable resource for the scientific community, particularly for young scientists and graduate and undergraduate students, inspiring more research in the vitally important field of environmental toxicity, molecular toxicology, and toxicogenomics.