Environmental Responses in Plants: Methods and Protocols

ISBN-13: 9781071622995 / Angielski

Paula Duque; D?ra Szakonyi

Spis treści

PART I      Tropisms and OTHER Developmental responses

1        Analysis of Plant Root Gravitropism

Richard Barker, Sarah Johns, Ralph Trane, and Simon Gilroy

 

2        Hydrotropism: Analysis of the Root Response to a Moisture Gradient

 Irene García-Maquilón, Jorge Lozano-Juste, Abdulwahed F. Alrefaei, and Pedro L. Rodriguez

 

3        Evaluating Root Mechanosensing Response in Rice

Tiago F. Lourenço, André M. Cordeiro, João Frazão, Nelson J.M. Saibo, and M. Margarida Oliveira

 

4        Physiological Analysis of Phototropic Responses to Blue and Red Light in Arabidopsis

Mathias Zeidler

 

5        Shoot Branching Phenotyping in Arabidopsis and Tomato

Ana Confraria, Aitor Muñoz-Gasca, Liliana Ferreira, Elena Baena-González, and Pilar Cubas

 

6        Ribo-Seq Method to Study Genome-Wide Translational Regulation in Plants

Hao Chen, Jose M. Alonso, and Anna N. Stepanova

 

PART II    Photoperiodism and Circadian Rythms

7        Assessing Flowering Time Under Different Photoperiods

Jesús Praena, Elizabeth van Veen, Rossana Henriques, and Reyes Benlloch

 

8        Analysis of Phytochrome-Dependent Seed Germination in Arabidopsis

Mathias Zeidler

 

9        Rhythmic Leaf and Cotyledon Movement Analysis

Ping Lou, Kathleen Greenham, and C. Robertson McClung

 

10    Assessing Plant Pigment Regulation in Circadian Experiments

Raquel Esteban, Víctor Resco de Dios, and José Ignacio García-Plazaola

 

11    Chemical perturbation of Chloroplast Ca2+ Dynamics in Arabidopsis thaliana Suspension Cell Cultures and Seedlings

Panfeng Yao, Steffen Vanneste, Lorella Navazio, Frank Van Breusegem, and Simon Stael

 

PART III   Abiotic Stress Responses

12    Screening for Abiotic Stress Response in Rice

Telma Fernandes, Fredilson Melo, Maria Beatriz Vieira, Tiago F. Lourenço, Chiara Pucciariello, Nelson J. M. Saibo, Isabel A. Abreu, and M. Margarida Oliveira

 

13    Etiolated Hypocotyls: A New System to Study the Impact of Abiotic Stress on Cell Expansion

Guiomar Martín and Paula Duque

 

14    Assessing Postgermination Development in Arabidopsis thaliana under Abiotic Stress

Rui Albuquerque-Martins, Alba Rodríguez Díez, Dóra Szakonyi, and Paula Duque

15    Assessing Abscisic Acid-Mediated Changes in Stomatal Aperture Through High-Quality Leaf Impressions

Alba Rodríguez Díez, Paula Duque, and Rossana Henriques

 

16    Evaluation of the Anti-Transpirant Activity of ABA-Receptor Agonists in Monocot and Eudicot Plants

Daniel Franco-Aragón, Irene García-Maquilón, Alfredo Manicardi, Pedro L. Rodríguez, and Jorge Lozano-Juste

 

17    Quantifying Phytohormones In Vivo with FRET Biosensors and the FRETENATOR Analysis Toolset

James H. Rowe, Annalisa Rizza, and Alexander M. Jones

 

18    Optical Imaging Resources for Crop Phenotyping and Stress Detection

Phatchareeya Waiphara, Cyril Bourgenot, Lindsey J Compton, and Ankush Prashar

 

PART IV   Plant Interactions WITH OTHER ORGANISMS

19    Analysis of Innate Immune Responses Against Pathogenic Bacteria in Arabidopsis, Tomato and Barley Marion Wenig, Kornelia Bauer, Miriam Lenk, and A. Corina Vlot

 

20    Performing Infection Assays of Sporisorium reilianum f. sp. Zeae in Maize

Mamoona Khan and Armin Djamei

 

21    A Method to Measure the Damage Caused by Cell-Sucking Herbivores

André F. Mira, Luís Marques, Sara Magalhães, and Leonor R. Rodrigues

 

22    RNA Isolation from Nematode-induced Feeding Sites in Arabidopsis Roots Using Laser Capture Microdissection

Muhammad Shahzad Anjam, Shahid Siddique, and Peter Marhavy

 

23    High-Throughput Profiling of Root-Associated Microbial Communities

Felix Getzke and Stéphane Hacquard

 

24    Analysis of Immunity-Related Oxidative Bursts by a Luminol-Based Assay

Bushra Saeed and Marco Trujillo

Blurb

This second edition describes up-to-date methodological approaches, ranging from physiological assays to imaging and molecular techniques, to study a wide variety of plant responses to environmental cues. Chapters are divided into four sections detailing protocols to investigate key biological processes underlying plant environmental responses in the model organisms Arabidopsis thaliana and Physcomitrella patens as well as in different crop species. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Environmental Responses in Plants, Second Edition aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.