Engineering Natural Product Biosynthesis

ISBN-13: 9781071622759 / Angielski / Miękka / 2023

Opis

This volume highlights natural products, molecular methods for identifying, and current trends in designing non-natural natural products. Chapters guide readers through protocols on heterologous expression techniques, gene disruption, modified pathway regulators, and in-vitro studies. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge, Engineering Natural Product Biosynthesis: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. Chapter 13 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Spis treści

1.       A bioinformatics workflow for investigating fungal biosynthetic gene clusters

Jorge C. Navarro-Muñoz and Jérôme Collemare

 

2.       Investigating Fungal Biosynthetic Pathways Using Heterologous Gene Expression - Aspergillus oryzae as a heterologous host

Kate M. J. de Mattos-Shipley, Colin M. Lazarus and Katherine Williams

 

3.       Investigating Fungal Biosynthetic Pathways Using Heterologous Gene Expression: Aspergillus nidulans as a Heterologous Host

Danielle A. Yee and Yi Tang

 

4.       Investigating Fungal Biosynthetic Pathways Using Heterologous Gene Expression: Fusarium sp. as a Heterologous Host

Mikkel Rank Nielsen, and Jens Laurids Sørensen

 

5.       Heterologous Expression of Fungal Biosynthetic Pathways in Aspergillus nidulans using episomal vectors

Indra Roux and Yit Heng Chooi

 

6.       Targeted Genetic Engineering via Agrobacterium-mediated transformation in Fusarium solani

Mikkel Rank Nielsen, Samba Evelyne Kabemba Kaniki, and Jens Laurids Sørensen

 

7.       Investigating Fungal Biosynthetic Pathways Using Pichia pastoris as a Heterologous Host

Zhilan Qian, Qi Liu, and Menghao Cai

 

8.       Evolutionary genome mining for the discovery and engineering of natural product biosynthesis

Marc G. Chevrette, Nelly Selem-Mojica, César Aguilar, Kristin Labby, Edder D. Bustos-Diaz, Jo Handelsman, and Francisco Barona-Gómez

 

9.       Inducing Global Expression of Actinobacterial Biosynthetic Gene Clusters

Meghan Pepler, Xiafei Zhang, Hindra, and Marie A. Elliot

 

10.   Engineering modular polyketide biosynthesis in Streptomyces using CRISPR/Cas: a practical guide

Jean-Malo Massicard, Li Su, Christophe Jacob and Kira J Weissman

 

11.   CRISPR/Cas9-Based Methods for Inactivating Actinobacterial Biosynthetic Genes and Elucidating Function

Audam Chhun and Fabrizio Alberti

 

12.   Understanding and Manipulating Assembly Line Biosynthesis by Heterologous Expression in Streptomyces

 

Lihan Zhang, Takayoshi Awakawa, and Ikuro Abe

 

13.   Heterologous expression, purification, and characterization of type II polyketide acyl carrier proteins

Grayson S. Hamrick, Casey H. Londerganm, and Louise K. Charkoudian

 

14.   Cyanobacterial Genome Sequencing, Annotation and Bioinformatics

Jonna Teikari, Martin Baunach, and Elke Dittmann

 

15.   Single crossover to inactivate target gene in cyanobacteria

Jaimie Gibbons, Liping Gu, Yeyan Qiu and Ruanbao Zhou

 

16.   Double crossover approach to inactivate target gene in cyanobacteria

Jaimie Gibbons, Liping Gu and Ruanbao Zhou

 

17.   Expression of Cyanobacterial Biosynthetic Gene Clusters in Escherichia coli

 Alescia Cullen, Matthew Jordan, and Brett A. Neilan

 

18.   Saccharomyces cerevisiae as a heterologous host for natural products

Maximilian Otto, Dany Liu, and Verena Siewers

 

19.   Investigating Plant Biosynthetic Pathways Using Heterologous Gene Expression: Yeast as a Heterologous Host

Shanhui Xu, Sheng Wu, and Yanran Li

 

20.   Rapid Combinatorial Co-Expression of Biosynthetic Genes by Transient Expression in the Plant Host Nicotiana benthamiana

Ling Chuang and Jakob Franke

 

21.   Optimized Tools and Methods for Methanotroph Genome Editing

Sreemoye Nath, Jessica M. Henard, and Calvin A. Henard

 

22.   Microarray-based screening of putative Hsp90 inhibitors predicted, and isolated from microorganisms

Anusha Kishore, Artem Fetter, and Carsten Zeilinger

 

23.   Isolation of Water-Soluble Metabolites from Marine Invertebrates and Microorganisms

Lamonielli F. Michaliski, Darlon I. Bernardi, and Roberto G. S. Berlinck

 

24.   Natural Product Investigation in Lichens: Extraction and HPLC analysis of Secondary Compounds in Mycobiont Cultures

Muthukumar Srinivasan, Karthik Shanmugam and Hariharan Gopalasamudram Neelakantan