Electrophysiology: Basics, Modern Approaches and Applications

ISBN-13: 9783319807041 / Angielski / Miękka / 2018 / 162 str.

Jurgen Rettinger; Silvia Schwarz; Wolfgang Schwarz

Opis

This textbook presents a broad overview of topics concerning cellular electrophysiology - covering topics ranging from bioelectric phenomena recognized as far back as ancient Egypt to popular topics on the dangers of electrosmog. Without sacrificing scientific precision, this clear and concise work presents on the one hand the different methods and applications, on the other hand the biophysical fundamentals of ion-channel and carrier proteins. Numerous and carefully selected illustrations and diagrams supplement the text, while questions at the end of each chapter allow readers to test their understanding. Each section also includes references to relevant original literature for further reading. The book offers a valuable resource for students of biology, chemistry and physics with a special interest in biophysics.

Spis treści

Chapter 1

1     Introduction

1.1   INTRODUCTORY OVERVIEW

1.2   HISTORY OF ELECTROPHYSIOLOGY

Chapter 2

2     Basics: Theory

2.1   ELECTRICAL CHARACTERISTICS OF BIOLOGICAL MEMBRANES

2.2   ION DISTRIBUTION AT BIOLOGICAL MEMBRANES

2.3   DONNAN DISTRIBUTION AND NERNST EQUATION

2.4   GOLDMAN-HODGKIN-KATZ EQUATION

Chapter 3

3.    Basics: Methods

3.1   RECORDING ELECTRICAL SIGNALS FROM BODY SURFACE

3.2   THE EXAMPLE (ECG)

3.3   RECORDING ELECTRICAL SIGNALS FROM TISSUE

3.4   RECORDING ELECTRICAL SIGNALS FROM SINGLE CELLS

3.5   APPLICATION OF THE VOLTAGE-CLAMP TECHNIQUE

3.6   THE PATCH-CLAMP TECHNIQUE

Chapter 4

4     Automated Electrophysiology

4.1   AUTOMATED TWO-ELECTRODE VOLTAGE CLAMP

4.2   AUTOMATED PATCH CLAMP

Chapter 5

5     Ion-selective Channels

5.1   GENERAL CHARACTERISTICS OF ION CHANNELS

5.2   SPECIFIC ION CHANNELS

Chapter 6

6     Theory of Excitability

6.1   THE HODGKIN-HUXLEY DESCRIPTION OF EXCITATION

6.2   CONTINUOUS AND SALTATORY CONDUCTION OF ACTION POTENTIALS

6.3   GENERATION AND TRANSMISSION OF ACTION POTENTIALS

6.4   SUMMARY OF THE DIFFERENT TYPES OF POTENTIALS

6.5   ACTION POTENTIAL IN NON-NERVE CELLS

Chapter 7

7     Carrier-mediated Transport

7.1   GENERAL CHARACTERISTICS OF CARRIERS

7.2   CARRIERS ARE LIKE CHANNELS WITH ALTERNATING GATES

Chapter 8

8     Examples of Application of Electrophysiology

8.1   STRUCTURE-FUNCTION RELATIONSHIPS OF CARRIER PROTEINS

8.2   STRUCTURE-FUNCTION RELATIONSHIPS OF ION CHANNELS

8.3   VIRAL ION CHANNELS

Appendix

A1.   THE GRAPH THEORY

A2.   INFLUENCE OF EXTERNAL ELECTRICAL AND MAGNETIC FIELDS

A2.1. MAGNETOSTATIC FIELDS

A3.   A LABORATORY COURSE:

Important Physical Units

List of Symbols and Abbreviations

Index

O autorze

Dr. phil.nat. Jürgen RETTINGER
Product Manager
Multi Channel Systems MCS GmbH
Aspenhaustrasse 21
72770 Reutlingen, Germany

Jürgen Rettinger received his doctor degree in physics at Goethe-University in Frankfurt. in 1995. His focus as group leader at Max-Planck-Institute of Biophysics in Frankfurt was research of structure-function relationships of ligand-gated ion channels and teaching electrophysiology at Goethe-University Frankfurt, Shanghai Institutes of Biological Sciences and Fudan-University Shanghai. Since 2008 Jürgen Rettinger is working with Multi Channel Systems responsible as product manager for electrophysiology.

Silvia SCHWARZ
Research Scientist
Shanghai Key Laboratory for Acupuncture Mechanism and Acupoint Function
Fudan University
Guoshoujing Rd. 199
Shanghai 201203, China

Silvia Schwarz finished her university education in biology and chemistry at Albert-Ludwigs-University Freiburg in 1974. Thereafter, she was educated and worked as teacher in biology and chemistry until 2008. Since then she works as research associate at Shanghai Research Center for Acupuncture and Meridians (Shanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function, Fudan Univ.). Silvia Schwarz’s main research focus is on antiviral effects of Chinese herbal drugs applying electrophysiological methods.

Prof. Dr. rer.nat. Wolfgang SCHWARZ 
Professor for Physics
Institut für Biophysik, Goethe-Universität Frankfurt
Max-von-Laue-Str. 1, 
60438 Frankfurt a.M., Germany
Shanghai Key Laboratory for Acupuncture Mechanism and Acupoint Function
Fudan University
Guoshoujing Rd. 199
Shanghai 201203, China

Wolfgang Schwarz received his doctor degree in physics at University of Saarland in 1975. Thereafter, he spent two years as postdoc at University of Washington Seattle working on characteristics of ion channels. In 1981 he habilitated in physiology at University of Saarland and received a position as head of a Cell Physiology Laboratory at Max-Planck-Institute of Biophysics Frankfurt. Wolfgang Schwarz’s main focus turned to structure-function and regulation of carrier transporters and teaching electrophysiology as professor for physics at Goethe-University Frankfurt, Shanghai Institutes of Biological Sciences and Fudan-University Shanghai.  Since 2004 Wolfgang Schwarz is head of the Cell Electrophysiology Laboratory of Shanghai Research Center for Acupuncture and Meridian (Shanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function, Fudan Univ.) and concurrent professor at Fudan University.