Effect of Statins and/or Gamma-Radiation on Tumor Cell Line

ISBN-13: 9786139896202 / Angielski / Miękka / 2018 / 196 str.

Ibrahim Abdelrahman

Opis

The present study was conducted to investigate the effect of Gamma-radiation alone and/or combined with cytotoxic drug (Simvastatin) on the viability and the life cycle of myeloma cells. In preliminary study, Seventeen groups were designed to select three doses of Gamma-radiation from eight doses and one dose of Simvastatin from six doses in addition to three control groups. The results indicated that the dose of Simvastatin (0.1miM/l) given 24hr prior to Gamma- irradiation(0.5Gy) elevated the percentage of cell death to 37.5% as compared to 4.81% in case of radiation alone. It was found that, simvastatin treatment before irradiation caused arresting the cells at G0/G1 and G2/M Phases as assessed by cell cycle analysis using flowcytometry. It is interesting to find that simvastatin treatment of P3NS1 cells induced an increase in the intracellular ROS production leading to decreasing the antioxidant enzymes activity with increasing the expression of P53, Bax and Caspase-3 gene except in case of the Bcl-2 gene where it was decreased.

O autorze

Abdelrahman, IbrahimMy Career Objective is research and development in Molecular Biology specially Molecular Cancer Biology and link this field with Radiation protection and primary prevention that I am looking forward to achieve spectacular achievement that serve my country and the whole world which will give me a chance to become a Nobel Laureate.