Effect of Preservatives on Mitochondrial DNA : Comparative study on the effect of preservatives on mitochondrial DNA of spider Hippasa agelenoides (Lycosidae: Araneae)

ISBN-13: 9783659255106 / Angielski / Miękka / 2012 / 64 str.

T. V. Vineeth Kumar; Remya James; Sebastian P. A.

Opis

Earlier morphological features were the only criteria for taxonomical classification. Following the proposal of the structure, physical and chemical properties of DNA in the earlier half of twentieth century, that marked the beginning of molecular taxonomy. Morphological features are results of gene expression; molecular taxonomy can deliver a more precise and more accurate classification. For this the nuclear material should maintain its integrity. Hence it is necessary to preserve DNA with minimal degradation. In this scenario taxonomical studies demand a preservative that causes less harm to the DNA. Our study aims at comparing the effect of three routinely used preservatives - acetone, 70% ethanol and 4% formaldehyde. By comparing their effects we would identify which one among the three has a dual role as a good preservative and pose minimum damage to the DNA. Here we also compared the feasibility of four different extraction methods (DNeasy, Quick Extract, Master Pure and CTAB)

O autorze

Mr. Vineeth Kumar T V is a research Scholar at Rajiv Gandhi Centre for Biotechnology. Since an Msc student he has been working on Molecular systematics and DNA barcoding of both Vertebrates and Invertebrates. He has good number of Article published and is currently an active researcher on Amphibian skin Peptides.