Ebolaviruses: Methods and Protocols

ISBN-13: 9781493971152 / Angielski / Twarda / 2017 / 397 str.

Thomas Hoenen; Allison Groseth

Opis

This vital book collects key methods that have supported advancements in the field of ebolavirus molecular biology given the pressing need for the advancement of techniques for diagnostics, the development of vaccines and antivirals, and for furthering our understanding of ebolavirus biology.

Recenzje

"This book comprises three review chapters on filoviruses and 27 protocol chapters. ... Scientists working on filoviruses and those wishing to enter the field will find a compendium of carefully written methods and protocols relevant to their work. ... What makes the book stand out from many other volumes in this series is the inclusion of the three review chapters, which by themselves earn the book a spot on the bookshelf of any scientist working in the field." (Christoph Wirblich, Doody's Book Reviews, January, 2018)

Spis treści

Part I: Introduction

 

1. Marburg and Ebola Viruses: A Look Back and Lessons for the Future

            Hans Dieter Klenk and Werner Slenczka

 

2. 40 Years of Ebolavirus Molecular Biology: Understanding a Novel Disease Agent through the Development and Application of New Technologies

            Allison Groseth and Thomas Hoenen

 

3. Ebolavirus: An Overview of Molecular and Clinical Pathogenesis<

            Veronica Vine, Dana P. Scott, and Heinz Feldmann

 

Part II: Studying Ebolaviruses Under Biosafety Level 2 Conditions

 

4. Production of Filovirus Glycoprotein-Pseudotyped Vesicular Stomatitis Virus for Study of Filovirus Entry Mechanisms

            Rachel B. Brouillette and Wendy Maury

 

5. Lentiviral Vectors Pseudotyped with Filoviral Glycoproteins

            Patrick L. Sinn, Jeremy E. Coffin, Natarajan Ayithan, Kathleen H. Holt, and Wendy Maury

 

6. Modeling Ebola Virus Genome Replication and Transcription with Minigenome Systems

            Tessa Cressey, Kristina Brauburger, and Elke Mühlberger

 

7. Quantification of RNA Content in Reconstituted Ebola Virus Nucleocapsids by Immunoprecipitation

            Logan Banadyga and Hideki Ebihara

 

8. Modeling Ebolavirus Budding with Virus-Like Particles

            Olivier Reynard and Mathieu Mateo

 

9. Modeling the Ebolavirus Life Cycle with Transcription and Replication-Competent Virus-Like Particle Assays

            Nadine Biedenkopf and Thomas Hoenen

 

10. Assays to Measure Suppression of Type I Interferon Responses by Filovirus VP35 Proteins

            Priya Luthra and Christopher Basler

 

11. Non-Radioactive Northern Blot Analysis to Detect Ebola Virus Minigenomic mRNA

            Kristina Brauburger, Tessa Cressey, and Elke Mühlberger

 

Part III: Studying Infectious Ebolaviruses In Vitro

 

12. A Semi-Automated High Throughput Microtitration Assay for Filoviruses

            Claire Marie Filone, David Miller, and Victoria Wahl-Jensen

 

13. Generation of Recombinant Ebola Viruses Using Reverse Genetics

            Allison Groseth

 

14. Luciferase-Expressing Ebolaviruses as Tools for Screening of Antivirals

            Thomas Hoenen

 

15. Live-Cell Imaging of Filoviruses

            Gordian Schudt, Olga Dolnik, and Stephan Becker

 

16. Assessment of Inhibition of Ebola Virus Progeny Production by Antiviral Compounds

            Darryl Falzarano

 

17. Analysis of the Cellular Stress Response During Ebola Virus Infection by Immunofluorescence

            Emily V. Nelson and Kristina M. Schmidt

 

18. Analyzing Apoptosis Induction and Evasion in Ebola Virus-Infected Cells

            Judith Olejnik and Emily V. Nelson

 

19. Electron Microscopy of Ebola Virus-Infected Cells

            Takeshi Noda

 

20. Validating the Inactivation Effectiveness of Chemicals on Ebola Virus

            Elaine Haddock and Friederike Feldmann

 

21. Visualizing Ebolavirus Particles Using Single-Particle Interferometric Reflectance Imaging Sensor (SP-IRIS)

            Erik P. Carter, Elif Ç. Seymour, Steven M. Scherr, George G. Daaboul, David S. Freedman, M. Selim Ünlü, and John H. Connor

 

Part IV: Studying Infectious Ebolaviruses In Vivo

 

22. Assessing Antiviral Countermeasures Using Mouse Models of Ebolavirus Infection

            Andrea Kroeker, Bryan D. Griffin, Xiangguo Qiu, and Gary Kobinger

 

23. Evaluation of Ebola Virus Countermeasures in Guinea Pigs

            Andrea Marzi

 

24. Evaluation of Medical Countermeasures against Ebolaviruses in Non-Human Primate Models

            Chad E. Mire and Thomas W. Geisbert

 

25. Quantification of Filovirus Glycoprotein-Specific Antibodies

            Wakako Furuyama, Hiroko Miyamoto, Reiko Yoshida, and Ayato Takada

 

26. Monitoring Innate Immune Gene Responses in the Hamster Model of Ebola Virus Disease by RT-PCR

            Marko Zivcec

 

Part V: Ebolaviruses in the Field

 

27. Real-Time and End Point PCR Diagnostics for Ebola Virus

            Allen Grolla

 

28. Production of Antigens for ELISA

            Robert W. Cross and Thomas G. Ksiazek

 

29. ELISA Methods for the Detection of Ebolavirus Infection

            Robert W. Cross and Thomas G. Ksiazek

 

30. Ebola Virus Field Sample Collection

            Brian R. Amman, Amy J. Schuh, and Jonathan S. Towner

Blurb

This vital book collects key methods that have supported advancements in the field of ebolavirus molecular biology given the pressing need for the advancement of techniques for diagnostics, the development of vaccines and antivirals, and for furthering our understanding of ebolavirus biology.  After an introduction, the volume delves into protocols for studying ebolavirus molecular biology under biosafety level 2 conditions, studying infectious ebolaviruses under biosafety level 4 conditions in vitro as well as in vivo, and working with ebolaviruses in the field.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Ebolaviruses: Methods and Protocols serves as a guide to the next generations of filovirus researchers and also helps to bring experts from other areas into the filovirus research arena.