Drug Design and Discovery: Methods and Protocols

ISBN-13: 9781493958245 / Angielski / Miękka / 2016 / 69 str.

Seetharama D. Satyanarayanajois

Opis

This volume includes work by leading experts who provide an in-depth view of key protocols used in drug discovery laboratories. It covers both classic and cutting-edge techniques and provides reproducible laboratory protocols and troubleshooting tips.

Recenzje

From the reviews:

"This volume is an easily accessible collection of protocols covering a broad range of different screening methodologies. ... The strong academic background of the authors makes this book highly recommendable to everyone involved in drug screening and has a particular appeal to an academic audience. ... It is highly recommended for all those who are performing drug screening or who aim to be doing so in the near future." (Robin Ketteler, British Journal of Clinical Pharmacology, June, 2011)

Spis treści

1. Virtual Screening for Lead Discovery

            Yat T. Tang and Garland R. Marshall

 

2. Computer-Aided Drug Discovery and Development

            Shuxing Zhang

 

3. Using Active Site Mapping and Receptor-Based Pharmacophore Tools: Prelude to Docking and De Novo/Fragment-Based Ligand Design

            Ashutosh Tripathi, J. Andrew Surface, and Glen E. Kellogg

 

4. Methods for Evaluation of Structural and Biological Properties of Anti-Invasive Natural Products

            Mudit Mudit, Mohammad Khanfar, Girish V. Shah, and Khalid A. El Sayed

 

5. Solid-Phase Peptide Synthesis Using Microwave Irradiation

            Justin K. Murray, Jennifer Aral, and Les P. Miranda

 

6. Fluorescent and Lanthanide Labeling for Ligand Screens, Assays, and Imaging

            Jatinder S. Josan, Channa R. De Silva, Byunghee Yoo, Ronald M. Lynch, Mark D. Pagel, Josef Vagner, and Victor J. Hruby

 

7. DNA-Directed Assembly Microarray for Protein and Small Molecule Inhibitor Screening

            Ng Jin Kiat, Fritz Simeon, Too Heng Phon, and Parayil Kumaran Ajikumar

 

8. Selection of Peptide Ligands for Human Placental Transcytosis Systems Using In vitro Phage Display

            Saleem Basha, Shruthi Vaidhyanathan, and Giovanni M. Pauletti

 

9. Optimization of the Tetrazolium Dye (MTT) Colorimetric Assay for Cellular Growth and Viability

            Paul W. Sylvester

 

10. Imaging NF- κB Signaling in Mice for Screening Anti-Cancer Drugs

            Delira Robbins and Yunfeng Zhao

 

11. Evaluation of Anticancer Agents Using Flow Cytometry Analysis of Cancer Stem Cells

            Vineet Gupta, Qian-Jin Zhang, and Yong-Yu Liu

 

12. Chemical Screening with Zebrafish Embryos

            Hanbing Zhong and Shuo Lin

 

13. iTRAQ^TM Labeling Coupled with LC-MALDI Mass Spectrometry for Monitoring Temporal Response of Colorectal Cancer Cells to Butyrate Treatment

            Hwee Tong Tan, Teck Kwang Lim, Maxey C.M. Chung, and Qingsong Lin

 

14. Heterotypic Cell Adhesion Assay for the Study of Cell Adhesion Inhibition

            Seetharama D. Satyanarayanajois, Sharon Ronald, and Jining Liu

 

15. Evaluation of Antibacterial Activity of Proteins and Peptides Using a Specific Animal Model for Wound Healing

            Ramar Perumal Samy, Maung Maung Thwin, Vincent T.K.Chow, Ho Bow, and  Ponnampalam Gopalakrishnakone

 

16. In vitro Immunogenicity Risk Assessment of Therapeutic Proteins in Preclinical Setting

            Puneet Gaitonde and Sathy V. Balu-Iyer

Blurb

Research in the pharmaceutical sciences and medicinal chemistry has taken an important new direction in the past two decades with a focus on large molecules, especially peptides and proteins, as well as DNA therapeutics.  In Drug Design and Discovery: Methods and Protocols, leading experts provide an in-depth view of key protocols that are commonly used in drug discovery laboratories.  Covering both classic and cutting-edge techniques, this volume explores computational docking, quantitative structure-activity relationship (QSAR), peptide synthesis, labeling of peptides and proteins with fluorescent labels, DNA-microarray, zebrafish model for drug screening, and other analytical screening and biological assays that are routinely used during the drug discovery process.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Thorough and accessible, Drug Design and Discovery: Methods and Protocols serve as a vital laboratory reference for pharmaceutical chemists, medicinal chemists, and pharmacologists as well as for molecular biologists.