Drosophila Oogenesis: Methods and Protocols

ISBN-13: 9781071629697 / Angielski / Twarda / 2023 / 457 str.

Michelle S. Giedt; Tina L. Tootle

Opis

This detailed volume compiles numerous methods to explore fly oogenesis. Beginning with updated protocols from isolating and staining the ovary to numerous imaging techniques and genetic protocols for cell-specific assessment and CRISPR-mediated mutagenesis, the volume continues by detailing techniques from assessing cytoskeletal structures to uncovering protein-protein interactions, with closing chapters exploring how Drosophila oogenesis can be used in the classroom and in outreach programs to increase interest in biomedical research, STEM education, and STEM careers. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Drosophila Oogenesis: Methods and Protocols serves as an ideal guide for both new and expert researchers working with this robust and versatile model organism.

Spis treści

1. The Vast Utility of Drosophila Oogenesis

            Michelle S. Giedt and Tina L. Tootle

 

2. Visualization and Quantification of Drosophila Larval Ovaries

            Alicia E. Rosales-Nieves, Miriam Marín-Menguiano, Alejandro Campoy-Lopez, and Acaimo González-Reyes

 

3. Dissection, Fixation, and Standard Staining of Adult Drosophila Ovaries

            Julie A. Merkle

 

4. Utilizing the FLP-Out System for Clonal RNAi Analysis in the Adult Drosophila Ovary

            Daniel N. Phipps, Amanda M. Powell, and Elizabeth T. Ables

 

5. Analysis of Physiological Control of Adult Drosophila Oogenesis by Inter-Organ Communication

            Lesley N. Weaver

 

6. Immunohistochemical Analysis of Nuclear Lamina Structures in the Drosophila Ovary Using CRISPR-Tagged Genes

            Tingting Duan, Felipe Rodriguez-Tirado, and Pamela K. Geyer

 

7. Visualizing Fusome Morphology via Tubulin Immunofluorescence in Drosophila Ovarian Germ Cells

            Anna E. Williams and Elizabeth T. Ables

 

8. Tracking Follicle Cell Development

            Adrianna Soriano, Christopher Petit, Savannah Ryan, and Jennifer C. Jemc

 

9. Optimized Fixation and Phalloidin Staining of Basally Localized F-Actin Networks in Collectively Migrating Follicle Cells

            Mitchell T. Anderson, Kristin Sherrard, and Sally Horne-Badovinac

 

10. Quantitative Image Analysis of Dynamic Cell Behaviors during Border Cell Migration

            Yujun Chen, Nirupama Kotian, and Jocelyn A. McDonald

 

11. Live Imaging of Nurse Cell Behavior in Late Stages of Drosophila Oogenesis

            Jonathan A. Jackson, Jasmin Imran Alsous, and Adam C. Martin

 

12. Visualizing Lipid Droplets in Drosophila Oogenesis

            Roger P. White and Michael A. Welte

 

13. Assessing Ovulation in Drosophila melanogaster

            Andrew Beard, Rebecca Oramas, and Jianjun Sun

 

14. A Low-Tech Flow Chamber for Live Imaging of Drosophila Egg Chambers during Drug Treatments

            Allison L. Zajac, Audrey M. Williams, and Sally Horne-Badovinac

 

15. Detection and Assessment of Wolbachia pipientis Infection

            Lindsay B.M. Nevalainen and Irene L.G. Newton

 

16. Measuring Transposable Element Activity in Adult Drosophila Ovaries

            Anikó Szabó, Péter Borkúti, Zoltán Kovács, Ildikó Kristó, Csilla Abonyi, and Péter Vilmos

 

17. Preparation of Drosophila Ovarioles for Single-Cell RNA Sequencing

            Nathaniel Meyer, Jobelle Peralta, and Todd Nystul

 

18. Chromatin Immunoprecipitation Experiments from Drosophila Ovaries

            Maria Sokolova and Maria K. Vartiainen

 

19. Detection of Actin in Nuclear Protein Fraction Isolated from Adult Drosophila Ovary

            Ildikó Kristó, Péter Borkúti, Zoltán Kovács, Anikó Szabó, Szilárd Szikora, and Péter Vilmos

 

20. STAMP: Spatio-Temporal Association Mapping of Proteins

            Yuanbing Zhang, Bo Zhang, and Ji-Long Liu

 

21. Using Drosophila Oogenesis in the Classroom to Increase Student Participation in Biomedical Research

            Jayson A. Cortes and Christina I. Swanson

 

22. Gaining Wings to FLY: Using Drosophila Oogenesis as an Entry Point for Citizen Scientists in Laboratory Research

            Dara M. Ruiz-Whalen, Christopher P. Aichele, Ebony R. Dyson, Katherine C. Gallen, Jennifer V. Stark, Jasmine A. Saunders, Jacqueline C. Simonet, Erin M. Ventresca, Isabela M. Fuentes, Nyellis Marmol, Emly Moise, Benjamin C. Neubert, Devon J. Riggs, Ava M. Self, Jennifer I. Alexander, Ernest Boamah, Amanda J. Browne, Iliana Correa, Maya J. Foster, Nicole Harrington, Troy J. Holiday, Ryan A. Henry, Eric H. Lee, Sheila M. Longo, Laurel D. Lorenz, Esteban Martinez, Anna Nikonova, Maria Radu, Shannon C. Smith, Lindsay A. Steele, Todd I. Strochlic, Nicholas F. Archer, Y. James Aykit, Adam J. Bolotsky, Megan Boyle, Jennifer Criollo, Oren Eldor, Gabriela Cruz, Valerie N. Fortuona, Shreeya D. Gounder, Nyim Greenwood, Kayla W. Ji, Aminah Johnson, Sophie Lara, Brianna Montanez, Maxwell Saurman, Tanu Singh, Daniel R. Smith, Catherine A. Stapf, Tarang Tondapu, Christina Tsiobikas, Raymond Habas, and Alana M. O’Reilly