Drosophila

ISBN-13: 9781071625439 / Angielski / Miękka / 2023

Opis

This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and cell lineage tracing, protein inhibition and tagging, optogenetic and optochemical control of tissue mechanics, AFM measurements, sample preparation for electron microscopy, live imaging of different tissues and organs, and quantitative image analysis. Written in the format of the highly successful Methods in Molecular Biology series, chapters include an introduction to their topic, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, Drosophila: Methods and Protocols, Third Edition serves as a useful and practical guide to new researchers and experts using Drosophila as a model system.Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Spis treści

                       

1.              Using FlyBase, a database of Drosophila genes and genetics

Victoria K. Jenkins, Aoife Larkin, Jim Thurmond and The FlyBase Consortium

2.              The Q-System: A versatile repressible binary expression system

Orsolya Fölsz, Chun-Chieh Lin, Darya Task, Olena Riabinina and Christopher J. Potter

3.              Resources and methods for the analysis of microRNA function in Drosophila

Sromana Mukherjee and Nicholas Sokol

4.              Analysis of single-cell transcriptome data in Drosophila

Schayan Yousefian, Maria Jelena Musillo and Josephine Bageritz                               

5.              Prime editing for precise genome engineering in Drosophila

Justin A. Bosch and Norbert Perrimon

6.              CRISPR/Cas9 mediated precise and efficient genome editing in Drosophila

Kevin G. Nyberg and Richard W. Carthew

7.              Tissue-specific CRISPR-Cas9 screening in Drosophila

Fillip Port and Michael Boutros

8.              CRISPR-based transcriptional activation in Drosophila

Yuting Han, Xinyi Lu, Yutong Li, Yuhao Qiu, Xizhi Dong, Xiaochen Li, Xu Si, Qingfei Liu, Jian-Quan Ni

9.              Tracing and manipulating Drosophila cell lineages based on CRISPR: CaSSA and CLADES

Jorge Garcia-Marques and Tzumin Lee

10.           Studying protein function using nanobodies and other protein binders in Drosophila

Katarzyna Lepeta, Milena Bauer, Gustavo Aguilar, Alessandra Vigano, Shinya Matsuda and Markus Affolter

11.           Anchor Away: A system for fast inhibition of proteins in Drosophila

Pablo Sanchez Bosch

12.           Tagging Drosophila proteins with genetically-encoded fluorophores

Jerome Avellaneda and Frank Schnorrer

13.           Optogenetic methods to control tissue mechanics in Drosophila

Daniel Krueger and Stefano De Renzis

14.           Optochemical control of cell contractility in Drosophila embryos

Deqing Kong and Jörg Großhans

15.           Stiffness measurement of Drosophila egg chambers by atomic force microscopy

Uwe Töpfer, Karla Yanín Guerra Santillán and Elisabeth Fischer-Friedrich

16.           Cultivation and live imaging of Drosophila imaginal discs

Natalie A. Dye

17.           Sample preparation and imaging of the pupal Drosophila abdominal epidermis 

Daiki Umetsu

18.           FlyClear: A tissue clearing technique for high-resolution microscopy of Drosophila

Marko Pende, Saiedeh Saghafi, Klaus Becker, Thomas Hummel and Hans-Ulrich Dodt

19.           Preparation of Drosophila tissues and organs for transmission electron microscopy

Olympia-Ekaterini Psathaki and Achim Paululat

20.           Segmentation and quantitative analysis of epithelial tissues

Benoit Aigouy and Benjamin Prud’homme

21.           Genetically-encoded sensors to study metabolism in Drosophila

Ellen McMullen, Helen Hertenstein, Stephan Müller and Stefanie Schirmeier