Part I General Topics and Transversal Methods in DH Technology
1. Overview of In Vitro and In Vivo Doubled Haploid Technologies Jose M. Seguí-Simarro, Nathanaël M.A. Jacquier, and Thomas Widiez
2. Applications of Doubled Haploids in Plant Breeding and Applied Research Jens Weyen
3. Species with Haploid or Doubled Haploid Protocols Jose M. Seguí-Simarro, Javier Belinchón Moreno, Marina Guillot Fernández, and Ricardo Mir Moreno
4. Analysis of Ploidy in Haploids and Doubled Haploids Sergio J. Ochatt and Jose M. Seguí-Simarro
5. Methods for Chromosome Doubling Mehran E. Shariatpanahi, Mohsen Niazian, and Behzad Ahmadi
Part II Doubled Haploids in Alliaceae
6. Doubled Haploid Onion (Allium cepa L.) Production via In Vitro Gynogenesis Ali Ramazan Alan
7. In Vitro Gynogenesis in Leek (Allium ampeloprasum L.) Fevziye Celebi-Toprak and Ali Ramazan Alan
Part III Doubled Haploids in Cereals
8. Barley Isolated Microspore Culture Luís Cistué and Begoña Echávarri
9. Site-Directed Mutagenesis in Barley using RNA-GUided Cas Endonucleases during Microspore-Derived Generation of Doubled Haploids Robert Eric Hoffie, Ingrid Otto, Hiroshi Hisano, and Jochen Kumlehn
10. Generation of Doubled Haploid Barley by Interspecific Pollination with Hordeum bulbosum Pooja Satpathy, Sara Audije de la Fuente, Vivian Ott, Andrea Müller, Heike Büchner, Diaa Eldin S. Daghma, and Jochen Kumlehn
11. Bread Wheat Doubled Haploid Production by Anther Culture Ana María Castillo, Isabel Valero-Rubira, Sandra Allué, Asunción Costar, and María Pilar Vallés
12. Unpollinated Ovaries Used to Produce Doubled Haploid Lines in Durum Wheat Olfa Ayed Slama and Hajer Slim Amara
13. In Vitro Anther Culture for Doubled Haploid Plant Production in Spelt Wheat Csaba Lantos and János Pauk
14. Production of Wheat Doubled Haploids through Intergeneric Hybridization with Maize Pierre Devaux
15. Isolation of Staged and Viable Maize Microspores for DH Production Philippe Vergne and Antoine Gaillard
16. Triticale Isolated Microspore Culture for Doubled Haploid Production Priti Maheshwari and John D. Laurie
17. Oat (Avena sativa L.) Anther Culture Marzena Warchoł, Kinga Dziurka, Ilona Czyczyło-Mysza, Katarzyna Juzoń, Izabela Marcińska, and Edyta Skrzypek
18. Oat Doubled Haploid Production through Wide Hybridization with Maize Edyta Skrzypek, Marzena Warchoł, Ilona Czyczyło-Mysza, Katarzyna Juzoń, Kinga Dziurka, and Izabela Marcińska
19. Anther Culture and Chromosome Doubling in Mediterranean Japonica Rice Isidre d’Hooghvorst, Irene Ferreres, and Salvador Nogués
20. High Throughtput Doubled Haploid Production for Indica Rice Breeding Swapan K. Tripathy
Subject Index List…
This title offers 62 chapters divided among three volumes covering the latest topics dealing with Doubled Haploid (DH) technology, as well as methods to produce DHs in different species through different in vivo and in vitro approaches. Volume 1 explores general topics and transversal methods in DH technology; DH production in two alliaceae, onion, and leek; and DH production in cereals such as barley, durum, bread, oat, and japonica rice. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Doubled Haploid Technology, Volume 1: General Topics, Alliaceae, Cereals is a valuable resource for scientists and researchers looking to learn more about this interesting and developing field.