Differences in Nuclear DNA Preservation in Human Skeletal Remains

ISBN-13: 9783838338828 / Angielski / Miękka / 2010 / 104 str.

Cassandra Kuba

Opis

The focus of this study is to explore differences in DNA preservation between adult and subadult remains and to determine if enough DNA is present to permit determination of family relatedness. The skeletal remains of eleven adults and seven subadults from two 19th century cemeteries from Indianapolis, Indiana, were tested using nuclear DNA analysis to determine sex, as well as Short Tandem Repeat (STR) primers to explore questions of familial relatedness. The results of the study conclude that biological age does not strongly influence the quantity of human DNA obtained or the quality of lower molecular weight DNA sequences. However, biological age did have an influence on the ability to amplify high molecular weight sequences. The nuclear DNA present in these samples was not preserved well enough to permit familial testing.

O autorze

Dr. Kuba received her Ph.D. in Anthropology from Arizona State University in 2006 and is an assistant professor of Anthropology in the Department of Justice, Law & Society at California University of Pennsylvania. Her research interests are in forensic anthropology, bioarchaeology, nonmetric analysis, and biological affiliation.