Diagnostic Molecular Biology

ISBN-13: 9780128028230 / Angielski / Miękka / 2019 / 472 str.

Chang-Hui Shen

Opis

Diagnostic Molecular Biology describes the fundamentals of molecular biology in a clear, concise manner to help readers comprehend this complex subject. Each technique is explained within its conceptual framework to enhance understanding, and current applications of clinical laboratory techniques are covered in detail. This targeted approach covers the principles of molecular biology, including the basic knowledge of nucleic acids, proteins, chromosomes, the basic techniques and instrumentations that are commonly used in the field of molecular biology with detailed procedures and explanations for each technique; and the applications of the principle and techniques currently employed in the clinical laboratory.

• Provides an understanding on how techniques are used to diagnosis at the molecular level
• Explains how to use information technology to communicate and assess results in the lab
• Places protocols into context with practical applications

Spis treści

Part I1. Nucleic Acids: DNA and RNA2. Nucleic Acid-Based Cellular Activities3. Gene Expression: Transcription of the Genetic Code4. Gene Expression: Translation of the Genetic Code5. The Genome

Part II6. Extraction and Purification of Nucleic Acids and Proteins7. Detection and Analysis of Nucleic Acids8. Quantification and Analysis of Proteins9. Amplification of Nucleic Acids10. Characterization of Nucleic Acids and Proteins11. Techniques in Sequencing12. Genome and Transcriptome Analysis

Part III13. Molecular Diagnosis of Chromosomal Disorders14. Molecular Diagnosis of Mutation and Inherited Diseases15. Molecular Diagnosis of Infectious Diseases16. Guidance for Molecular Clinical Laboratory

O autorze

Chang-Hui Shen, PhD, is a Professor of Biology at the College of Staten Island, City University of New York, New York. Dr. Shen graduated from the National Chung-Hsing University, Taiwan, with both a BS and MS, earning his PhD from the University of Edinburgh, UK. He previously was a visiting fellow of the National Institutes of Health before taking a position at the College of Staten Island, City University of New York.

Dr. Shen's research focuses on understanding the mechanism of gene expression, with an emphasis on the roles of transcriptional coactivators in gene activation. He also pursues such topics as the regulation of programmed cell death through major cellular signal transduction pathways and transcriptional networks in the cell, as well as epigenetic regulation of neurodegenerative diseases. Dr. Shen has been teaching Diagnostic Molecular Biology for more than 15 years.