Diagnostic Cytogenetics

ISBN-13: 9783642478130 / Angielski / Miękka / 2014 / 460 str.

Rolf-Dieter Wegner

Opis

Following a section on tissue culture, chromosome staining and basic information about karyotyping, this text presents nomenclature and quality standards, as well as protocols of relevance to comprehensive cytogenetic diagnostics.

Spis treści

Part I Classical Cytogenetics: 01 Tissue Culture, Friedel Wenzel; 02 Chromosome staining, Angelika Köhler; 03 Karyotyping and Data Interpretation, Karsten Held; 04 Documentation, Peter Miny, Rolf-Dieter Wegner; Part II Postnatal Diagnosis: 05 Peripheral Blood, Iris Bartels; 06 Establishment of permanent growing lymphoblastoid cell lines, Heidemarie Neitzel; 07 Solid tissues, Regine Schubert and Gesa Schwanitz; 08 Cells from urine sample, Hannelore Körner, Henrike Dia, Christiane Bommer; 09 Classical and molecular cytogenetics of tumor cells, Brigitte Schlegelberger, Simone Metzke, Svetlana Harder, Reina Zühlke-Jenisch,Yanming Zhang, Reiner Siebert; 10 Cytogenetics of Meiotic Cells, R. Johannisson; Part III Prenatal Diagnosis: 11 Prenatal Diagnosis - an introduction, Rolf-Dieter Wegner; 12 Amniotic fluid cell analysis, Ingo Kennerknecht, Mahmoud Djalali, Gotthold Barbi, Walter Just, Walther Vogel; 13 Chorionic villi sampling, Rolf-Dieter Wegner and Holger Toennies; Part IV Special Applications: 14 Diagnosis of chromosomal instability syndromes, Rolf-Dieter Wegner, Markus Stumm; 15 Flow Cytometric Testing for Syndromes with Chromosomal Instability and for Aplastic Anemia and Related Hematological Disorders, Detlev Schindler and Holger Hoehn; 16 Cell fusion, Markus Stumm and Rolf-Dieter Wegner; 17 Origin of Trisomies, Gesa Schwanitz and Thomas Eggermann; Part V Molecular Cytogenetics: 18 Fluorescence in situ hybridization, Gesa Schwanitz and Regine Schubert; 19 Fluorescence in situ Hybridization (FISH) Analysis in Human Sperm Cells, Evelyn Ko and Rene Martin; 20 Microdissection and Reverse Chromosome Painting, G. Senger, I. Chudoba, J. Weimer and U. Claussen; 21 Comparative genomic hybridization (CGH), Traudl Henn, Oskar A. Haas; Part VI Techniques in Development: 22 Fetal cells in maternal blood, Dorothee Gänshirt, Henk S.P.Garritsen, Wolfgang Holzgreve; 23 Spectral Karyotyping in Clinical and Tumor Cytogenetics, Evelin Schröck, Yuval Garini, Michael Köhler andThomas Ried; 24 Chromosome analysis by multiplex-FISH (M-FISH), Michael R. Speicher

Blurb

In addition to the traditional cytogenetics still used as the basic methodology for everyday clinical diagnosis, new molecular cytogenetic techniques provide a useful basis for routine diagnosis. Flourescence in situ hybridization (FISH) has become a standard technique, and comparative genomic hybridization (CGH), sprectral karyotyping (SKY), and multi-color FISH have shown their potential for diagnostic purposes. Following a section on tissue culture, chromosome staining and basic information about karyotyping, nomenclature and quality standards, protocols of relevance for comprehensive cytogenetic diagnostics are presented.