Diagnostic Bacteriology: Methods and Protocols

ISBN-13: 9781493970353 / Angielski / Twarda / 2017 / 264 str.

Kimberly A. Bishop-Lilly

Opis

This volume provides a comprehensive collection of protocols on molecular diagnostics of bacteria that will suit the needs of molecular biologists, clinical laboratorians, and physician scientists alike.

Spis treści

1. Whole Genome Enrichment using RNA Probes and Sequencing of Chlamydia Trachomatis Directly from Clinical Samples

Amanda Claire Brown and Mette T. Christiansen

 

2. Characterization of Sinus Microbiota by 16S Sequencing from Swabs

Thad W. Vickery, Jennifer M. Kofonow, and Vijay R. Ramakrishnan

 

3. Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR)

Véronique Wuyts, Wesley Mattheus, Nancy H.C. Roosens, Kathleen Marchal, Sophie Bertrand, and Sigrid C.J. De Keersmaecker

 

4. Detection of Helicobacter pylori DNA in Formalin-fixed Paraffin Embedded Gastric Biopsies using Laser Micro-dissection and qPCR

Maria Fernanda Loayza Villa, Valeria Liliana Herrera Sevilla, and Nicolás Vivar Díaz

 

5. Mycobacterial Load Assay

Stephen H. Gillespie, Wilber Sabiiti, and Katarina Oravcova

 

6. Defining Diagnostic Biomarkers using Shotgun Proteomics and MALDI-TOF Mass Spectrometry

Jean Armengaud

 

7. Detection and Typing of ‘Candidatus Phytoplasma’ spp. in Host DNA Extracts Using Oligonucleotide-Coupled Fluorescent Microspheres

Edel Pérez-López, Christine Hammond, Chrystel Olivier, and Tim J. Dumonceaux

 

8. Detection of Helicobacter Pylori in the Gastric Mucosa by Fluorescence in vivo Hybridization

Silvia Fontenete, Marina Leite, Ceu Figueiredo, Paul Cos, and Nuno F. Azevedo

 

9. Rapid Antibiotic Susceptibility Testing for Urinary Tract Infections

Anja Mezger, Mats Nilsson, and Dan I. Andersson

 

10. Detection and Differentiation of Lyme Spirochetes and other Tick-borne Pathogens from Blood using real-time PCR with Molecular Beacons

Samantha Schlachter, Kamfai Chan, Salvatore A. E. Marras, and Nikhat Parveen

 

11. Methods for Real-time PCR-based Diagnosis of Chlamydia Pneumoniae, Chlamydia Psittaci and Chlamydia Abortus Infections in an Opened Molecular Diagnostic Platform

Onya Opota, René Brouillet, Gilbert Greub, and Katia Jaton

 

12. Real-time PCR to Identify Staphylococci and Assay for Virulence from Blood

Charles E. Okolie

 

13. Multiplex Peptide Nucleic Acid Fluorescence in situ Hybridization (PNA-FISH) for Diagnosis of Bacterial Vaginosis

Antonio Machado and Nuno Cerca

 

14. A Closed-tube Loop-Mediated Isothermal Amplification Assay for the Visual Endpoint Detection of Brucella spp. and Mycobacterium Avium Subsp. Paratuberculosis

Marcos D. Trangoni, Andrea K. Gioffré, and Silvio L. Cravero

 

15. Highly Specific Ligation-Dependent Microarray Detection of Single Nucleotide Polymorphisms

Noa Wolff and Ivan Barišić

 

16. Multilocus Sequence Typing (MLST) for Cronobacter spp.

 

17. Diagnostic Bacteriology: Raman Spectroscopy

Rebecca L. Pavlicek, Nicole J. Crane, Meron Ghebremedhin, Katherine Cilwa, and Eric A. Elster

Blurb

This volume provides a comprehensive collection of protocols on molecular diagnostics of bacteria that will suit the needs of molecular biologists, clinical laboratorians, and physician scientists alike. Chapters detail common bacterial pathogens, protocols that can be applied to diverse or even unknown pathogens, digital PCR, next generation sequencing, and bioinformatic analyses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Diagnostic Bacteriology: Methods and Protocols delivers a wide range of assay types all on the cutting edge of diagnostic bacteriology.