Developmental Toxicology: Methods and Protocols

ISBN-13: 9781617798665 / Angielski / Twarda / 2012 / 471 str.

Craig Harris; Jason M. Hansen

Opis

The discipline of developmental toxicology is an integration of concepts, models, and methodologies based heavily on the superimposition of toxicology principles upon the science of developmental biology. The science of developmental toxicology also borrows from other research areas that are concerned with regulation of cell growth, migration, differentiation and cell death, as such are central to the study of stem cells, cancer, and chronic diseases. In Developmental Toxicology: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study developmental toxicology highlighting the evolution of methods from classical teratology approaches to the dynamic, state-of-the-art molecular methods, systems biology, and next generation models and procedures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Developmental Toxicology: Methods and Protocols is a valuable resource to those planning experiments to investigate consequences of environmental, nutritional, or chemical effects caused during development.

Spis treści

1. Volume Overview - Introduction

Craig Harris

 2. In Vivo Models of Developmental Toxicology

Jason M. Hansen

 3. Caenorhabditis Elegans as a Model in Developmental Toxicology

Windy A. Boyd, Marjolein V. Smith, and Jonathan H. Freedman

 4. Zebrafish Embryo Developmental Toxicology Assay

Julieta M. Panzica-Kelly, Cindy X. Zhang, Karen Augustine-Rauch

 5. Gene Knockdown by Morpholino-modified Oligonucleotides in the Zebrafish (Danio rerio) Model: Applications for Developmental Toxicology 

Alicia R. Timme-Laragy, Sibel I. Karchner, Mark E. Hahn

 6. An Amphibian Model for Studies of Developmental Reproductive Toxicity

Cecilia Berg

 7. Avian Models in Teratology and Developmental Toxicology

Susan M. Smith, George R. Flentke, Ana Garic

 8. Overview of In Vitro Models in Developmental Toxicology

Craig Harris

 9. Primary Cell and Micromass Culture in Assessing Developmental Toxicity

M Pratten, Bhavesh K. Ahir, H. Smith-Hurst, S Memon, P Mutch & P.Cumberland

 10. Embryonic Stem Cell Test (EST): Stem cell Use in Predicting Developmental Cardiotoxicity and Osteotoxicity

Béatrice Kuske, Polina Y. Pulyanina, Nicole I. zur Nieden

 11. Mouse Embryonic Stem Cell Adherent Cell Differentiation and Cytotoxicity (ACDC) Assay

Marianne Barrier, Kelly Chandler, Susan Jeffay, Maria Hoopes, Tom Knudsen, Sid Hunter

 12. The Murine Limb Bud in Culture as an In Vitro Teratogenicity Test System

France-Hélène Paradis, Chunwei Huang and Barbara F. Hales

 13. Rodent Whole Embryo Culture

Craig Harris

 14. Rabbit Whole Embryo Culture

Valeris A. Marshall and Edward W. Carney

 15. Assessment of Xenobiotic Biotransformation Including Reactive Oxygen Species Generation in the Embryo using Benzene as an Example

Helen J. Renaud, Allison Rutter, and Louise M. Winn

 16. Methodological Approaches to Cytochrome P450 Profiling in Embryos

Jared V. Goldstone and John J. Stegeman

 17. Analysis of Nrf2-Mediated Transcriptional Induction of Antioxidant Response in Early Embryos

Shao-yu Chen

 18. Regulation and Control of AP-1 Binding Activity in Embryotoxicity

Terence R.S. Ozolinš

 19. Thioredoxin Redox Status Assessment During Embryonic Development:  The Redox Western

Jason M. Hansen

 20. Methods for the Determination of Plasma or Tissue Glutathione Levels

Trent E. Tipple and Lynette K. Rogers

21. Oxidative Stress, Thiols, and Redox Profiles

Craig Harris and Jason M. Hansen

22. A Review of Toxicogenomic Approaches in Developmental Toxicology

Joshua F. Robinson, Jeroen L.A. Pennings, Aldert H. Piersma

23. Epigenetic Approaches and Methods in Developmental Toxicology: Role of Inhibition in Teratogenic Events

Elena Menegola, Graziella Cappelletti and Francesca Di Renzo

24. DNA Methylation Screening and Analysis

Karilyn E. Sant, Muna S. Nahar, and Dana C. Dolinoy

25. Assessment of Histiotrophic Nutrition using Fluorescent Probes

Jeffrey Ambroso and Craig Harris

26. Diabetic Embryopathy

Ulf J. Eriksson and Parri Wentzel

 27. Gene Expression Analysis in Developing Embryos: In situ Hybridization

Siew-Ging Gong

 28. Assessment of Gross Fetal Malformations:  The Modernized Wilson Technique and Skeletal Staining

BRobert E. Seegmiller, Nathan Cook, Korance Goodwin, and Timothy Leishman