Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols

ISBN-13: 9781071609767 / Angielski / Miękka / 2021

Carroll, David J.

Opis

This detailed second edition presents a wide variety of marine invertebrate model systems, from cephalochordata to holothurians, along with novel experimental protocols for taking advantage of their unique properties. The techniques range from culturing the organisms to modifying their DNA. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, Second Edition is an ideal guide for researchers working with these versatile organisms and for furthering our understanding of fundamental biological questions.

Spis treści

1. Laboratory Culture and Mutagenesis of Amphioxus (Branchiostoma floridae)

            Linda Z. Holland and Guang Li

 

2. Handling and Manipulation of Gametes and Embryos of the Annelidan Worm Pseudopotamilla occelata

            Ryusaku Deguchi and Takeshi Nakano

 

3. The Starfish Asterina pectinifera: Collection and Maintenance of Adults and Rearing and Metamorphosis of Larvae

            Naoyuki Murabe, Ei-ichi Okumura, Kazuyoshi Chiba, Enako Hosoda, Susumu Ikegami, and Takeo Kishimoto

 

4. Experimental Tools to Study Regeneration in the Sea Anemone Nematostella vectensis

            Aldine R. Amiel and Eric Röttinger

 

5. Staining and Tracking Methods for Studying Sponge Cell Dynamics

            Carole Borchiellini, Sandie M. Degnan, Emilie Le Goff, Caroline Rocher, Amélie Vernale, Stephen Baghdiguian, Nina Séjourné, Florent Marschal, André Le Bivic, Nelly Godefroy, Bernard M. Degnan, and Emmanuelle Renard

 

6. Microscopy Studies of Placozoans

            Carolyn L. Smith, Tatiana D. Mayorova, Christine A. Winters, Thomas S. Reese, Sally P. Leys, and Andreas Heyland

 

7. Identification of SH2 Domain-Mediated Protein Interactions that Operate at Fertilization in the Sea Star Patiria miniata

            Lauren Bates, Emily Wiseman, Jamie Kitson, and David J. Carroll

 

8. Marine Nemertean Worms for Immunoblotting Studies of Oocyte Aging

            Stephen A. Stricker

 

9. Recovery of Sea Star Egg Cell Surface Proteins Released at Fertilization

            Emily Wiseman, Lauren Bates, and David J. Carroll

 

10. Quantifying Cell Proliferation During Regeneration of Aquatic Worms

            Eduardo E. Zattara and B. Duygu Özpolat

 

11. In Situ Hybridization Techniques in the Homoscleromorph Sponge Oscarella lobularis

            Laura Fierro-Constain, Caroline Rocher, Florent Marschal, Quentin Schenkelaars, Nina Sejourné, Carole Borchiellini, and Emmanuelle Renard

 

12. Methodology for Whole Mount and Fluorescent RNA In Situ Hybridization in Echinoderms: Single, Double, and Beyond

            Margherita Perillo, Periklis Paganos, Maxwell Spurrell, Maria I. Arnone, and Gary M. Wessel

 

13. Gene Editing in the Ascidian Phallusia mammillata and Tail Nerve Cord Formation

            Alex McDougall, Celine Hebras, Isa Gomes, and Remi Dumollard

 

14. Transcriptomic Analysis in the Sea Anemone Nematostella vectensis

            Jacob F. Warner and Eric Röttinger

 

15. RNA Interference on Regenerating Holothurian Gut Tissues

            Miosotis Alicea-Delgado, Samir A. Bello-Melo, and José E. García-Arrarás

 

16. ATAC-Seq for Assaying Chromatin Accessibility Protocol Using Echinoderm Embryos

            Marta Silvia Magri, Danila Voronov, Jovana Ranđelović, Claudia Cuomo, Jose Luis Gómez-Skarmeta, and Maria I. Arnone

 

17. Usage of the Sea Urchin Hemicentrotus pulcherrimus Database, HpBase

            Sonoko Kinjo, Masato Kiyomoto, Takashi Yamamoto, Kazuho Ikeo, and Shunsuke Yaguchi

 

18. Functional Genomic Studies of Trichoplax adhaerens Neuronal Activity

            Julia Gauberg, Adriano Senatore, and Andreas Heyland

 

19. A Bioinformatics Tutorial for Comparative Development Genomics in Diverse Meiofauna

            Joseph L. Sevigny, Jon L. Norenburg, and Francesca Leasi