Derivation of Lymphocytes from Human induced Pluripotent Stem Cells

ISBN-13: 9783659697241 / Angielski / Miękka / 2015 / 100 str.

Ma Chao

Opis

Human pluripotent stem cells have the potential to produce essentially unlimited numbers of mature and functional blood lineage populations to study human hematopoiesis. Particularly, human induced pluripotent stem cells (hiPSCs) have the advantage to provide a source of autologous transplantable blood cell populations suitable for treatment of patient specific hematological diseases. The book describes three projects of research aiming to derive human lymphocytes from hiPSCs. The overall generation of human lymphocytes (B cell, T cell and NK cell) from hiPSCs is explored in Project I. In Project II and III, based on the derivation of NK cells, two human immunodeficiency disease models (X-SCID and WAS), both caused by specific somatic gene mutation, are established using human pluripotent stem cells. The ultimate goal of this research is to use hiPSCs to study the normal development of human lymphocytes in vitro, as well as model human immunodeficiency diseases by combining with gene therapy methods, thus providing a novel approach for immunotherapy.

O autorze

Born in a rural area of Shandong province in China, I obtained my D.V.M. degree in 2011. Under God's mighty hands, I pursed graduate study in Dr. Dan Kaufman's lab, working with human pluripotent stem cells to study human hematopoiesis and lymphocyte development. In 2014, I was awarded a M.S. degree in the University of Minnesota, Twin Cities, USA.