Dendritic Cells: Methods and Protocols

ISBN-13: 9781071629376 / Angielski

Vanja Sisirak

Opis

This detailed volume provides methods that can be used to study dendritic cell (DC) ontogeny, isolation, migration, and functions. After an introduction to murine and human DC subsets and their unique transcriptional, phenotypic, and functional properties, the book continues with sections covering in vivo studies, in vitro differentiation, enrichment, functional characterization, as well as Omics approaches to study dendritic cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Dendritic Cells: Methods and Protocols is an ideal guide to familiarize readers with the current state of the art techniques to investigate these vital cells.

Spis treści

Part I: Overview on Mouse and Human Dendritic Cells

 

1. Origin, Phenotype, and Function of Mouse Dendritic Cell Subsets

            Dorothée Duluc and Vanja Sisirak

 

2. Phenotypes and Functions of Human Dendritic Cell Subsets Particularly in the Tumor Microenvironment

            Candice Sakref, Nathalie Bendriss-Vermare, and Jenny Valladeau-Guilemond

 

Part II: In Vivo Study of Dendritic Cells

 

3. In Vivo Tracking of Dendritic Cell Migration

            Michio Tomura

 

4. In Vivo Analysis of Dendritic Cell Clonality

            Mar Cabeza-Cabrerizo

 

Part III: In Vitro Differentiation of Dendritic Cells

 

5. In Vitro Generation of Murine Bone Marrow-Derived Dendritic Cells

            Yohan Gerber-Ferder, Pierre Bourdely, Mathias Vetillard, Pierre Guermonprez, and Julie Helft

 

6. In Vitro Generation of Murine Dendritic Cells from Hoxb8-Immortalized Hematopoietic Progenitors

            Hans Häcker

 

7. In Vitro Generation of Murine CD8α⁺ DEC205⁺ XCR1⁺ Cross-Presenting Dendritic Cells from Bone Marrow-Derived Hematopoietic Progenitors

            Margaret E. Kirkling and Boris Reizis

 

8. In Vitro Generation of Human Dendritic Cell Subsets from CD34⁺ Cord Blood Progenitors

            Pierre Bourdely, Roberto Savoldelli, Mathias Vetillard, Giorgio Anselmi, Julie Helft, and Pierre Guermonprez

 

9. In Vitro Generation of Human Cross-Presenting Type 1 Conventional Dendritic Cells (cDC1s) and Plasmacytoid Dendritic Cells (pDCs)

            Xinlong Luo, Sreekumar Balan, Catharina Arnold-Schrauf, and Marc Dalod

 

10. Culture System Allowing the Simultaneous Differentiation of Human Monocytes into Dendritic Cells and Macrophages Using M-CSF, IL-4, and TNF-a

            Javiera Villar, Alice Coillard, Corné van Roessel, and Elodie Segura

 

11. Clonal Analysis of Human Dendritic Cell Progenitors Using a Stromal Cell Culture

            Kang Liu, Jaeyop Lee, and Thomas Luh

 

Part IV: Enrichment of Dendritic Cells

 

12. Enrichment of Large Numbers of Splenic Mouse Dendritic Cells after Injection of Flt3L-Producing Tumor Cells

            Pauline Santa, Anaïs Roubertie, Séverine Loizon, Anne Garreau, Amandine Ferriere, Dorothée Duluc, and Vanja Sisirak

 

13. Isolation and Identification of Dendritic Cell Subsets from Human and Mouse Tumors

            Yamila Rocca, Aurélien Voissière, Jenny Valladeau-Guilemond, and Nathalie Bendriss-Vermare

 

Part V: Functional Characterization of Dendritic Cells

 

14. Optimized Non-Viral Gene Disruption in Primary Murine and Human Myeloid Cells

            Emily C. Freund, Simone M. Haag, Benjamin Haley, and Aditya Murthy

 

15. Monitoring the Interaction between Dendritic Cells and T Cells In Vivo with LIPSTIC

            Giulia Pasqual, Aleksey Chudnovskiy, and Gabriel D. Victora

 

16. Characterization of Dendritic Cell Metabolism by Flow Cytometry

            Eline C. Brombacher, Thiago A. Patente, Marjolein Quik, and Bart Everts

 

17. In Vivo and In Vitro Assay to Address Dendritic Cells Antigen Cross-Presenting Capacity

            Pengju Ou, Lifen Wen, Hai Ni, and Cliff. Y. Yang

 

18. Assays to Detect Cross-Dressing by Dendritic Cells In Vivo and In Vitro

            Alok Das Mohapatra and Pramod K. Srivastava

 

19. Assessing the Ability of Human Dendritic Cells to Stimulate Naive CD4⁺ and CD8⁺ T Cells

            Alice Coillard, Tsing-Lee Tang-Huau, and Elodie Segura

 

20. In Vitro and In Vivo Assays to Evaluate Dendritic Cell Phagocytic Capacity

            Lucie Maisonneuve and Bénédicte Manoury

 

21. Tracking Plasmacytoid Dendritic Cell Response to Physical Contact with Infected Cells

            Margarida Sá Ribeiro, Garima Joshi, Elodie Décembre, Célia Nuovo, Adrien Bosseboeuf, Alicia Bellomo, Manon Venet, Sonia Assil, and Marlène Dreux

 

Part VI: Omic Approaches to Study Dendritic Cells

 

22. Harnessing Single Cell RNA Sequencing to Identify Dendritic Cell Types, Characterize Their Biological States, and Infer Their Activation Trajectory

            Ammar Sabir Cheema, Kaibo Duan, Marc Dalod, and Thien-Phong Vu Manh

 

23. Characterization of Developmental Trajectories of Dendritic Cell Hematopoiesis through Single Cell RNA Sequencing Methods

            Regine J. Dress and Florent Ginhoux