Dendritic Cell Protocols

ISBN-13: 9781493980925 / Angielski / Miękka / 2018 / 322 str.

Elodie Segura; Nobuyuki Onai

Spis treści

Review of Mouse and Human Dendritic Cell Subsets.- In Vitro Generation of Human Xcr1⁺ Dendritic Cells from Cd34⁺ Hematopoietic Progenitors.- Derivation and Utilization Of Functional Cd8⁺ Dendritic Cell Lines.- Isolation of Dendritic Cell Progenitor and Bone Marrow Progenitor Cells from Mouse.-  The Isolation and Enrichment of Large Numbers of Highly Purified Mouse Spleen Dendritic Cell Populations and their In Vitro Equivalents.- Dendritic Cell Subsets Purification from Human Tonsils and Lymph Nodes.- Isolation and Identification of Conventional Dendritic Cell Subsets from the Intestine of Mice and Men.- Isolation of Human Skin Dendritic Cell Subsets.- Isolation of Mouse Dendritic Cell Subsets and Macrophages from Skin.- Isolation of Conventional Dendritic Cells from Mouse Lungs.- Purification of Human Dendritic Cell Subsets from Peripheral Blood.- Protocols for the Identification and Isolation of Antigen-Presenting Cells in Human and Mouse Tissues.- Measurement of Export to the Cytosol in Dendritic Cells Using a Cytofluorimetry-Based Assay.- Cross-Presentation Assay for Human Dendritic Cells.- Analysis of Intracellular Trafficking of Dendritic Cell Receptors for Antigen Targeting.- Characterization of Dendritic Cell Subsets through Gene Expression Analysis.- In Vivo Ablation of a Dendritic Cell Subset Expressing a Chemokine Receptor, Xcr1.- In Vivo Analysis Of Intestinal Mononuclear Phagocytes.- In Vivo Imaging Of Cutaneous Dcs In Mice.- Analysis of Dendritic Cell Function by Clec9a-Dtr Transgenic Mice.- Analysis of Dc Functions Using Cd205-Dtr Knock-In Mice.- Generation of Humanized Mice for Analysis of Human Dendritic Cells.