Decellularized Scaffolds and Organogenesis: Methods and Protocols

ISBN-13: 9781493976553 / Angielski / Twarda / 2018 / 347 str.

Kursad Turksen

Opis

While knowledge of how to grow and differentiate cells in culture has dramatically improved over time, the book addresses the challenges of how to instruct particular cells of interest to recognize and respond to their environment so as to proliferate, differentiate, and function for restoration of original tissue and organ form and function.

Spis treści

1. Decellularization Methods for Scaffold Fabrication

            Sweta K. Gupta and Narayan C. Mishra

 

2. Decellularized Liver Scaffold for Liver Regeneration

            Wei Yang, Renpei Xia, Yujun Zhang, Hongyu Zhang, and Lianhua Bai

 

3. Wharton’s Jelly Matrix Decellularization for Tissue Engineering Applications

            Gabriel L. Converse, Dandan Li, Eric E. Buse, Richard A. Hopkins, and Omar S. Aljitawi

 

4. 3D-Decellularized Native Extra Cellular Matrix Scaffold for In Vitro Culture Expansion of Human Wharton Jelly Mesenchymal Stem Cells (hWJ MSCs)

            Balasubramanian Sundaram, Anne George Cherian, and Sanjay Kumar

 

5. Decellularized Iliotibial Band Recolonized with Allogenic Homotopic Fibroblasts or Bone Marrow-Derived Mesenchymal Stromal Cells

            Clemens Gögele, Silke Schwarz, Benjamin Ondruschka, Niels Hammer, and Gundula Schulze-Tanzil

 

6. Preparation of Cell-Derived Decellularized Matrices Mimicking Native ECM During the Osteogenesis and Adipogenesis of Mesenchymal Stem Cells

            Takashi Hoshiba, Naoki Kawazoe, and Guoping Chen

 

7. Mouse Skeletal Muscle Decellularization

            Martina Piccoli, Caterina Trevisan, Edoardo Maghin, Chiara Franzin, and Michela Pozzobon

 

8. Extracellular Matrix from Whole Porcine Heart Decellularization for Cardiac Tissue Engineering

            Matthew J. Hodgson, Christopher C. Knutson, Nima Momtahan, and Alonzo D. Cook

 

9. In-Lab Manufacturing of Decellularized Rat Renal Scaffold for Kidney Bioengineering

            Andrea Peloso, Antonio Citro, Valeria Corradetti, Szandra Branbilla, Graziano Oldani, Francesco Calabrese, Tommaso Dominioni, Marcello Maestri, and Lorenzo Cobianchi

 

10. Glutaraldehyde Crosslinking Modification of Decellularized Rat Kidney Scaffolds

            Yaling Yu, Li Liu, Jianse Zhang, Zairong Wei, and Jin Mei

 

11. Preparation of Scaffolds from Decellularized Testicular Matrix

            Yoni Baert and Ellen Goossens

 

12. Decellularization of Bovine Small Intestinal Submucosa

            Mahmut Parmaksiz, A. Eser Elçin, and Y. Murat Elçin

 

13. Decellularization and Recellularization of Cartilage

            Catherine A. Bautista and Bahar Bilgen

 

14. A Protocol to Prepare Decellularized Stem Cell Matrix for Rejuvenation of Cell Expansion and Cartilage Regeneration

            Jingting Li and Ming Pei

 

15. Tracheal Cartilage Isolation and Decellularization

            Zachary Galliger and Angela Panoskaltsis-Mortari

 

16. Protocols for Rat Uterus Isolation and Decellularization: Applications for Uterus Tissue Engineering and 3D Cell Culturing

            Arvind Manikantan Padma, Tom Tristan Tiemann, Ahmed Baker Alshaikh, Randa Akouri, Min Jong Song, and Mats Hellström

 

17. Application of Decellularized Tissue Scaffolds in Ovarian Tissue Transplantation

            Enes Taylan and Kutluk Oktay

 

18. Composite Bioscaffolds Incorporating Decellularized ECM as a Cell-Instructive Component within Hydrogels as In Vitro Models and Cell Delivery Systems

            Arthi Shridhar, Elizabeth Gillies, Brian G. Amsden, and Lauren E. Flynn

 

19. 3D-Printing Composite Polycaprolactone-Decellularized Bone Matrix Scaffolds for Bone Tissue Engineering Applications

            Alexandra N. Rindone, Ethan Nyberg, and Warren L. Grayson

 

20. Decellularization of Large Tendon Specimens: Combination of Manually Performed Freeze‑Thaw Cycles and Detergent Treatment

            Susanne Pauline Roth, Ina Erbe, and Janina Burk

 

21. Decellularized Bone Matrix Scaffold for Bone Regeneration

            Guobao Chen and Yonggang Lv

 

22. Preparation of Decellularized Liver Scaffolds and Recellularized Liver Grafts

            Yibin Chen, Sharon Geerts, Maria Jaramillo, and Basak E. Uygun

 

23. Decellularization of Liver and Organogenesis in Rats

            Nana Shirakigawa and Hiroyuki Ijima

 

24. Simple and Quick Method to Obtain a Decellularized, Functional Liver Bioscaffold

            Matteo Ghiringhelli, Alessandro Zenobi, Stefano Brizzola, Fulvio Gandolfi, Valentino Bontempo, Sandro Rossi, Tiziana A.L. Brevini, and Fabio Acocella

 

25. Liver Bioengineering Using Decellularized Whole-Liver Scaffolds

            Iris Plá Palacín, Pilar Sainz Arnal, Sara Morini, Manuel Almeida, and Pedro M. Baptista

 

26. Decellularization of Intact Lung Tissue through Vasculature and Airways Using Negative and Positive Pressure

            Steven Skolasinski and Angela Panoskaltsis-Mortari

 

27. Alveolar Rhabdomyosarcoma Decellularization

            Michela Pozzobon, Mattia Saggioro, Stefania D’Agostino, Gianni Bisogno, Maurizio Muraca, and Piergiorgio Gamba

Blurb

This practical, hands-on volume examines the use of decellularized tissues and organs as biologically-active scaffolds by providing numerous approaches from experts in the field. While knowledge of how to grow and differentiate cells in culture has dramatically improved over time, the book addresses the challenges of how to instruct particular cells of interest to recognize and respond to their environment so as to proliferate, differentiate, and function for restoration of original tissue and organ form and function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and easy to use, Decellularized Scaffolds and Organogenesis: Methods and Protocols share novel approaches and insights that will provide new opportunities for those already in the field or moving to enter the field.