Storage and Viability Assessment of Date Palm Pollen
Maryam, Muhammad J. Jaskani, and Summar A. Naqvi
2
In Vitro Conservation of Date Palm Tissue Cultures
Shawky A. Bekheet
3
Cryopreservation of Date Palm Pro-embryonic Masses Using the D Cryo-Plate Technique
Mohammad Salma and Florent Engelmann
4
In Vitro Cryopreservation of Date Palm Caulogenic Meristems
Lotfi Fki, Olfa Chkir, Walid Kriaa, Ameni Nasri, Emna Baklouti, Raja B. Masmoudi, Alain Rival, oureddine Drira, and Bart Panis
5
In Vitro Conservation of Date Palm Shoot-Tip Explants and Callus Cultures under Minimal-Growth Conditions
Maiada M. El-Dawayati
6
In Vitro Conservation of Date Palm Somatic Embryos Using Growth Retardant Conditions
Mona M. Hassan
7
Encapsulation of Date Palm Somatic Embryos: Synthetic Seeds
Shawky A. Bekheet
Part II Molecular Analysis of In Vitro Cultures
8
Evaluation of Clonal Fidelity of Micropropagated Date Palm by Random Amplified Polymorphic DNA (RAPD)
Arpan Modi, Bhavesh Gajera, Naraynan Subhash, and Nitish Kumar
9
Molecular Identification of Fungal Contamination in Date Palm Tissue Cultures
Mohammed H. Abass
Part III Genetic Diversity and Cultivar Identity
10
Genetic Diversity Analysis of Date Palm Using Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) Mahesh K. Mahatma, Vishal S. Srivashtav, and Sanjay Jha
11
Date Palm Genetic Diversity Analysis Using Microsatellite Polymorphism
Hussam S.M. Khierallah, Saleh M. Bader, Alladin Hamwieh, and Michael Baum
12
Assessing Date Palm Genetic Diversity Using Different Molecular Markers
Mohamed A.M. Atia, Mahmoud M. Sakr, and Sami S. Adawy
13
Molecular Analysis of Date Palm Genetic Diversity Using Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR)
Sherif F. El Sharabasy and Khaled A. Soliman
14
Determining Phylogenetic Relationships among Date Palm Cultivars Using Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) Markers
Nadia Haider
15
Genotyping and Molecular Identification of Date Palm Cultivars Using Inter Simple Sequence Repeat (ISSR) Markers
Basim M. Ayesh
16
Molecular Identification of Date Palm Cultivars Using random amplified polymorphic DNA (RAPD) Markers
Nasser S. Al-Khalifah and A.E. Shanavaskhan
Part IV Gender Identification
17
Early Sex Identification in Date Palm by Male Specific Sequence-Characterized Amplified Region (SCAR) markers
Pushpa Kharb and Charu Mitra
18
Gender Identification in Date Palm Using Molecular Markers
Faisal S. Awan, Maryam, Muhammad J. Jaskani, and Bushra Sadia
19
Development of Sex-Specific PCR-based Markers in Date Palm
Mohamed A.M. Atia, Mahmoud M. Sakr, Morad M. Mokhtar, and Sami S. Adawy
20
Date Palm Sex Differentiation Based on Fluorescence In Situ Hybridization (FISH)
Mohamed A.M. Atia, Sami S. Adawy, and Hanaiya A. El-Itriby
Part V Genomics
21
Characterization and Amplification of Gene-Based Simple Sequence Repeats (SSR) Markers in Date Palm
Yongli Zhao, Manjunath Keremane, Channapatna S. Prakash, and Guohao He
22
Mitochondrial Molecular Markers for Resistance to Bayoud Disease in Date Palm
Amgad A. Saleh, Anwar H. Sharafaddin, Mahmoud H. El_Komy, Yasser E. Ibrahim, Younis K. Hamad, and Younis Y. Molan
23
Analysis of Expressed Sequence Tags (EST) in Date Palm
Sulieman A. Al-Faifi, Hussein M. Migdadi, Salem S. Algamdi, Mohammad Altaf Khan, Rashid S. Al-Obeed, Megahed H. Ammar, and Jerenj Jakse
24
Development of Genomic Simple Sequence Repeats (SSR) by Enrichment Libraries in Date Palm
Sulieman A. Al-Faifi, Hussein M. Migdadi, Salem S. Algamdi, Mohammad Altaf Khan, Rashid S. Al-Obeed, Megahed H. Ammar, and Jerenj Jakse
25
MicroRNA Expression in Multistage Date Fruit Development
Wanfei Liu, Chengqi Xin, Jun Yu, and Hasan Awad Aljohi
Part VI Proteomics
26
Proteome of Abiotic Stress Tolerance in Date Palm
Haddad A. El Rabey
27
Proteomics of Development and Germination of Date Palm Zygotic Embryos
Besma Sghaier-Hammami, Noureddine Drira, and Jesús V. Jorrín-Novo
28
Date Fruit Proteomics during Development and Ripening Stages
Claudius Marondedze
This two-volume book is a valuable resource to students, researchers, scientists, commercial producers, consultants and policymakers interested in agriculture or plant sciences particularly in date palm biotechnology. Chapters in Date Palm Biotechnology Protocols: Volume 2: Germplasm Conservation and Molecular Breeding guides readers through methods and protocols on germplasm in vitro conservation, molecular analysis of in vitro cultures, genetic diversity, cultivar identity, gender identification, genomics, and proteomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Date Palm Biotechnology Protocols: Volume 2: Germplasm Conservation and Molecular Breeding aims to supplement the previous volume and to provide precise stepwise protocols in the field of date palm biotechnology.