DNA and RNA Profiling in Human Blood: Methods and Protocols

ISBN-13: 9781627039130 / Angielski / Miękka / 2015 / 341 str.

Peter Bugert

Opis

Blood samples have consistently proven to be a key source of genetic material for a wide variety of diagnostic or research purposes. In DNA and RNA Profiling in Human Blood: Methods and Protocols, leading international experts contribute both established and recently developed protocols for complex and high-throughput DNA and RNA profiling. Divided into two thorough sections, the volume concentrates on DNA profiling for blood cell antigens through methods on high-throughput multiplex approaches and SNP typing, along with RNA profiling in blood cells addressing certain blood cell types such as platelets, reticulocytes, and megakaryocytes. Written in the highly successful Methods in Molecular Biology series format, all of the chapters include brief introductions on the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as the Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA and RNA Profiling in Human Blood: Methods and Protocols is an ideal guide to the molecular profiling approaches that have opened up this broad field of research and have shown great promise in the further identifying of disease markers in blood."

Spis treści

Part I. DNA Profiling for Blood Cell Antigens PCR-Elisa for High-Throughput Blood Group Genotyping Maryse St-Louise Single Base Extension in Multiplex Blood Group Genotyping Gregory A Denomme Real-Time PCR Assays for High-Throughput Blood Group Genotyping Fernando Araujo Real-Time PCR Assays for High-Throughput Human Platelet Antigen Typing Simon E. McBride Multiplex ABO-Genotyping by Minisequencing Gianmarco Ferri and Susi Pelotti Multiplex Genotyping for Thrombophilia-Associated SNPs by Universal Bead Arrays Susan Bortolin Pyrosequencing of Toll-Like Receptor Polymorphisms of Functional Relevance Parviz Ahmad-Nejad Multiplex HLA-Typing by Pyrosequencing Ying Lu, Julian Boehm, Lynn Nichol, Massimo Trucco and Steven Ringquist High-Throughput Multiplex HLA-Typing by Ligase Detection Reaction (LDR) and Universal Array (UA) Approach Clarissa Consolandi Medium- to High-Troughput SNP Genotyping Using VeraCode Microbeads Charles H. Lin, Joanne M Yeakley, Timothy K. McDaniel, and Richard Shen The Use of Maternal Plasma for Prenatal RhD Blood Group Genotyping Kirstin Finning, Pete Martin, and Geoff Daniels Part II. RNA Profiling in Blood Cells Nanoliter High-Throughput PCR for DNA and RNA Profiling Colin J. Brenan, Douglas Roberts, and James Hurley RNA Stabilization of Peripheral Blood and Profiling by Bead Chip Analysis Svenja Debey-Pascher, Daniela Eggle, and Joachim L. Schultze RNA Profiling in Peripheral Blood Cells by Fluorescent Differential Display PCR Martin Steinau and Mangalathu S. Rajeevan cDNA Amplification by SMART PCR and Suppressive-Substractive Hybridization (SSH)-PCR Andrew Hillman, Eimear Dunne, and Dermot Kenny Transcript Profiling of Human Platelets Using Microarray and Serial Analysis of Gene Expression (SAGE) Dmitri V. Gnatenko, John J. Dunn, John Schwedes, Wadie F. Bahou Genome-Wide Platelet RNA Profiling in Clinical Samples Angelika Schedel and Nina Rolf PCR Based Amplification of Platelet mRNA Sequences obtained from Small-Scale Platelet Samples Jutta M. Rox, Jens Müller, and Bernd Pötzsch MicroRNA Profiling of Megakaryocytes Ramiro Garzon Serial Analysis of Gene Expression Adapted for Downsized Extracts (SAGE/SADE) Analysis in Reticulocytes Béatrice Bonafoux and Thérèse Commes Real-Time PCR Analysis for Blood Cell Lineage Specific Markers Louise Edvardsson and Tor Olofsson Monitoring the Immune Response Using Real-Time PCR Patrick Stordeur