DNA Viruses: Methods and Protocols

ISBN-13: 9781627038102 / Angielski / Miękka / 2014 / 498 str.

Paul M Lieberman

Opis

A compendium of readily reproducible and novel methods to manipulate DNA viruses and characterize their varied biological properties. The authors emphasize techniques for viral detection and genetics, but also include methods for structure determination, gene expression, replication, pathogenesis, complex cellular models, recombinant genetics, and computational/systems approaches. Wide-ranging and highly practical, DNA Viruses: Methods and Protocols will stimulate new directions in virology research with its novel strategies for engineering viral vectors in gene therapy, and its advanced approaches for detecting viruses in human disease.

Recenzje

"Good presentation...laboratories working with DNA viruses will find ample useful material...." - Doody's Health Sciences Book Review Journal

Spis treści

Part I. Viral Detection Viral Detection Feng Wang-Johanning and Gary L. Johanning Quantitative Detection of Epstein-Barr Virus DNA in Clinical Specimens by Rapid Real-Time PCR Targeting a Highly Conserved Region of EBNA-1 Servi J. C. Stevens, Sandra A. W. M. Verkuijlen, and Jaap M. Middeldorp Profiling of Epstein-Barr Virus Latent RNA Expression in Clinical Specimens by Gene-Specific Multiprimed cDNA Synthesis and PCR Servi J. C. Stevens, Antoinette A. T. P. Brink, and Jaap M. Middeldorp Quantitative Detection of Viral Gene Expression in Populations of Epstein-Barr Virus-Infected Cells In Vivo Donna R. Hochberg and David A. Thorley-Lawson Detection and Quantification of the Rare Latently Infected Cell Undergoing Herpes Simplex Virus Transcriptional Activation in the Nervous System In Vivo Nancy M. Sawtell Reporter Cell Lines for the Detection of Herpes Simplex Viruses Szu-Hao Kung Part II. Virus Structure and Imaging Unraveling the Architecture of Viruses by High-Resolution Atomic Force Microscopy Alexander J. Malkin, Marco Plomp, and Alexander McPherson Studying the Structure of Large Viruses With Multiresolution Imaging Carmen San Martín Herpes Simplex Virus-Cell Interactions Studied by Low-Fading Contrasted Immunofluorescence Helle Lone Jensen and Bodil Norrild Herpes Simplex Virus-Cell Interactions Studied by Immunogold Cryosection Electron Microscopy Helle Lone Jensen and Bodil Norrild FTIR Microscopy Detection of Cells Infected With Viruses Vitaly Erukhimovitch, Marina Talyshinsky, Yelena Souprun, and Mahmoud Huleihel Part III. Virus Entry The JC Virus-Like Particle Overlay Assay Hirofumi Sawa and Rika Komagome Analysis of Fusion Using a Virus-Free Cell Fusion Assay Marisa P. McShane and Richard Longnecker Pseudovirions asSpecific Tools for Investigation of Virus Interactions With Cells Martin Sapp and Hans-Christoph Selinka Part IV. Gene Expression Simultaneous In Situ Detection of RNA, DNA, and Protein Using Tyramide-Coupled Immunofluorescence Brian A. Van Tine, Thomas R. Broker, and Louise T. Chow Identification and Characterization of Herpesviral Immediate-Early Genes Yan Yuan Part V. Replication and Genome Maintenance Methods for Measuring the Replication and Segregation of Epstein-Barr Virus-Based Plasmids Priya Kapoor and Lori Frappier DNA Affinity Purification of Epstein-Barr Virus OriP-Binding Proteins Constandache Atanasiu, Larissa Lezina, and Paul M. Lieberman Part VI. Pathogenesis Pre-B-Cell Colony Formation Assay Masato Ikeda and Richard Longnecker Luciferase Real-Time Bioluminescence Imaging for the Study of Viral Pathogenesis Gary D. Luker and David A. Leib Part VII. Complex Cell Systems Culturing Primary and Transformed Neuronal Cells for Studying Pseudorabies Virus Infection Toh Hean Ch'ng, E. Alexander Flood, and Lynn William Enquist Human Papillomavirus Type 31 Life Cycle: Methods for Study Using Tissue Culture Models Frauke Fehrmann and Laimonis A. Laimins Part VIII. Recombinant Genetics Molecular Genetics of Herpesviruses: A Recombinant Technology Approach Jason S. Knight, Subhash C. Verma, Ke Lan, and Erle S. Robertson Molecular Genetics of DNA Viruses: Recombinant Virus Technology Bernhard Neuhierl and Henri-Jacques Delecluse Genetic Analysis of Cytomegalovirus by Shuttle Mutagenesis Manfred Lee and Fenyong Liu Construction of a Gene Inactivation Library for Bovine herpesvirus 1 Using Infectious Clone Technology Timothy J. Mahony, Fiona M. McCarthy, Jennifer L. Gravel, and Peter L. Young Selective Sile

Blurb

Recently, modern laboratory techniques have been applied with great success to DNA viruses, an important viral class that shares basic survival strategies and mechanisms of pathogenesis, and also provides model systems for the investigation of basic biological processes. In DNA Viruses: Methods and Protocols, a panel of expert academic and biomedical researchers describe in detail the innovative methods they have developed to manipulate DNA viruses and characterize their varied biological properties. The authors emphasize techniques for viral detection and genetics, but also include methods for structure determination, gene expression, replication, pathogenesis, complex cellular models, recombinant genetics, and computational/systems approaches. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Wide-ranging and highly practical, DNA Viruses: Methods and Protocols will stimulate new directions in research virology with its novel strategies for engineering viral vectors in gene therapy, and its advanced approaches for detecting viruses in human disease.