DNA Replication: Methods and Protocols

ISBN-13: 9781493956609 / Angielski / Miękka / 2016 / 701 str.

Sonya Vengrova; Jacob Z. Dalgaard

Opis

Since the discovery of its structure, the field of DNA replication has expanded to cover a vast number of experimental systems. Here, expert researchers present techniques used to investigate DNA replication, focusing on recent technological developments.

Spis treści

Part I: Reviews 1. DNA Replication Initiation Stephen J. Aves 2. DNA Replication Fork Proteins Ulrich Hübscher 3. Random and Site-Specific Replication Termination Jacob Z. Dalgaard, Trevor Eydmann, Milana Koulintchenko, Suha Sayrac, Sonya Vengrova, and Tomoko Yamada-Inagawa 4. Checkpoint Regulation of DNA Replication Erik Boye, Henriette C. Skjølberg, and Beáta Grallert 5. Introduction to Molecular Combing: Genomics, DNA Replication and Cancer John Herrick and Aaron Bensimon Part II: Methods for Analysis of Replication of a Single Locus 6. Replication Initiation Point Mapping: Approach and Implications Sapna Das-Bradoo and Anja-Katrin Bielinsky 7. Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis Larry D. Mesner, Pieter A. Dijkwel, and Joyce L. Hamlin 8. Topological Analysis of Plasmid DNA Replication Intermediates Using Two-Dimensional Agarose Gels Olivier Hyrien 9. Analysis of Telomeric DNA Replication Using Neutral-Alkaline Two-Dimensional Gel Electrophoresis Svetlana Makovets 10. Chromatin Immunoprecipitation of Replication Factors Moving with the Replication Fork Jordan B. Rapp, Alison B. Ansbach, Chiaki Noguchi, and Eishi Noguchi 11. Density Transfer as a Method to Analyze the Progression of DNA Replication Forks José Antonio Tercero 12. High-Resolution Mapping of Points of Site-Specific Replication Stalling Sonya Vengrova and Jacob Z. Dalgaard 13. DNA Replication in Nucleus-Free Xenopus Egg Extracts Ronald Lebofsky, Tatsuro Takahashi, and Johannes C.Walter Part III: Genome-Wide Analysis Methods 14. ChIP-Chip to Analyze the Binding of Replication Proteins to Chromatin Using Oligonucleotide DNA Microarrays Christopher J. Viggiani, Jennifer G. Aparicio, and Oscar M. Aparicio 15. Analyzing Origin Activation Patterns by Copy Number Change Experiments Miruthubashini Raveendranathan and Anja-Katrin Bielinsky 16. Detection of Replication Origins Using Comparative Genomics and Recombinational ARS Assay Conrad A. Nieduszynski and Anne D. Donaldson 17. Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes Larry D. Mesner and Joyce L. Hamlin 18. Application of Alkaline Sucrose Gradient Centrifugation in the Analysis of DNA Replication after DNA Damage Sascha Raschke, Jun Guan, and George Iliakis Part IV: Biochemistry and Biophysics Methods 19. Isolation of Recombinant DNA Elongation Proteins Barbara Bohacek, Elena Ferrari, and Ulrich Hübscher 20. In vitro Assays for Studying Helicase Activities Jeong-Hoon Kim and Yeon-Soo Seo 21. The Use of 2-Aminopurine Fluorescence to Study DNA Polymerase Function Linda J. Reha-Krantz 22. Single-Molecule Observation of Prokaryotic DNA Replication Nathan A. Tanner and Antoine M. van Oijen Part V: Cell Biology and Genetic Methods 23. Visualization of DNA Replication Sites in Mammalian Nuclei Daniela S. Dimitrova 24. Cell Cycle Synchrony for Analysis of S. pombe DNA Replication Douglas D. Luche and Susan L. Forsburg 25. Measuring DNA Content by Flow Cytometry in Fission

Blurb

Since the discovery of DNA structure and throughout the ensuing "DNA era," the field of DNA replication has expanded to cover a vast number of experimental systems. In DNA Replication: Methods and Protocols, expert researchers present a collection of techniques and approaches used to investigate DNA replication with an emphasis on the most recent technological developments.  Beginning with several informative introductory review chapters, this extensive volume is organized for clarity while fully encouraging innovation by the mixing of methods to create new techniques.  Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, DNA Replication: Methods and Protocols provides an excellent tool for both established laboratories and individuals new to this exciting field of research.