DNA Replication: Methods and Protocols

ISBN-13: 9781493925957 / Angielski / Twarda / 2015 / 295 str.

Sonya Vengrova; Jacob Dalgaard

Opis

Updated and revised, this thorough volume covers a range of methods focusing on systems, including mammalian, yeast, bacterial and archaeal. This second edition of DNA Replication: Methods and Protocols describes approaches to analyze whole genomes to single molecules, as well as both in vivo and in vitro experiments. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, DNA Replication: Methods and Protocols, Second Edition provides a collections of methods intended for newcomers to this research field and for established laboratories.

Spis treści

1. A High-Throughput Confocal Fluorescence Microscopy Platform to Study DNA Replication Stress in Yeast Cells

Nikko P. Torres and Grant W. Brown

 

2. Microscopy Techniques to Examine DNA Replication in Fission Yeast

Marc D. Green, Sarah A. Sabatinos, and Susan L. Forsburg

 

3. High-Resolution Analysis of Mammalian DNA Replication Units

Vadim O. Chagin, Marius Reinhart, and M. Cristina Cardoso

 

4. Analysing the Dynamics of DNA Replication In Mammalian Cells Using DNA Combing

Marta Bialic, Vincent Coulon, Marjorie Drac, Thierry Gostan, and Etienne Schwob

 

5. Measuring DNA Content by Flow Cytometry in Fission Yeast

Sarah A. Sabatinos and Susan L. Forsburg

 

6. Incorporation of Thymidine Analogs for Studying Replication Kinetics In Fission Yeast

Nicholas Rhind

 

7. Edu Incorporation for FACS and Microscopy Analysis of DNA Replication in Budding Yeast

Nicolas Talarek, Julie Petit, Elisabeth Gueydon, and Etienne Schwob

 

8. Determination Of Deoxyribonucleoside Triphosphate Concentrations in Yeast Cells by Strong Anion-Exchange High-Performance Liquid Chromatography Coupled with Ultraviolet Detection

Shaodong Jia, Lisette Marjavaara, Robert Buckland, Sushma Sharma, and Andrei Chabes

 

9. Measuring Ribonucleotide Incorporation Into DNA In Vitro and In Vivo

Anders R. Clausen, Jessica S. Williams, and Thomas A. Kunkel

 

10. Detection And Sequencing Of Okazaki Fragments In S. Cerevisiae

Duncan J. Smith, Tejas Yadav, and Lestyn Whitehouse

 

11. Chip-Seq To Analyze The Binding Of Replication Proteins To Chromatin

A. Zachary Ostrow, Christopher J. Viggiani, Jennifer G. Aparicio, and Oscar M. Aparicio

 

12. Chromatin Immunoprecipitation To Detect DNA Replication And Repair Factors

Mariana C. Gadaleta, Osamu Iwasaki, Chiaki Noguchi, Ken-Ichi Noma, and Eishi Noguchi

 

13. Molecular Genetic Methods To Study DNA Replication Protein Function In Haloferax Volcanii, a Model Archaeal Organism

Xavier Giroux and Stuart A. Macneill

 

14. Single-Molecule Observation Of Prokaryotic DNA Replication

Hylkje J. Geertsema, Karl E. Duderstadt, and Antoine M. Van Oijen

 

15. Analysing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast

Saed Mohebi, Sarah A. E. Lambert, and Antony M. Carr

 

16. Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis

Larry D. Mesner, Pieter A. Dijkwel, and Joyce L. Hamlin

 

17. Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes

Larry D. Mesner and Joyce L. Hamlin