DNA Nanotechnology: Methods and Protocols

ISBN-13: 9781493985814 / Angielski / Twarda / 2018 / 334 str.

Giampaolo Zuccheri

Spis treści

1. DNA Nanotechnology: From the Pub to Information-based Chemistry

Nadrian C. Seeman

 

2. The Synthesis of Designer DNA

Rachel C. Little, Colette J Whitefield, Elmer M. Tuite, and Andrew R. Pike

 

3. Synthesis of DNA-based Nanowires

Alexander Kotlyar

  4. Preparation of Anti-miR PNAs for Drug Development and Nanomedicine

Alex Manicardi, Roberto Gambari, Luisa de Cola, and Roberto Corradini

  5. Loading of PNA and other Molecular Payloads on Inorganic Nanostructures for Theranostics

Alessandro Bertucci, Simone Silvestrini, Roberto Corradini, and Luisa De Cola

 

6. Design and Characterization of pH-triggered DNA Nanoswitches and Nanodevices Based on DNA Triplex Structures

Andrea Idili and Francesco Ricci

 

7. Characterizing Surface-immobilized DNA Structures and Devices Using a Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D)

Katherine E. Dunn, Martin A. Trefzer, Steven Johnson, and Andy M. Tyrrell

 

8. Hybridization Chain Reaction Design and Biosensor Implementation

Andrea Miti

 

9. Ultrasensitive Detection of Metal Ions with DNA Nanostructure

Mingshu Xiao, Xiangmeng Qu, Li Li, and Hao Pei

 

10. Spatially Resolved Peptide-DNA Nanoassemblages for Biomarker Detection; A Synergy of DNA Directed Immobilization and Nanografting

Abimbola Feyisara Adedeji, Elena Ambrosetti, Loredana Casalis, and Matteo Castronovo

  11. LSPR Detection of Nucleic Acids on Nanoparticle Monolayers

Sophie Thamm, Andrea Csàki, and Wolfgang Fritzsche

 

12. DNA-templated Silver Nanoclusters for DNA Methylation Detection

Hanie Ahmadzade Kermani, Morteza Hosseini, Mehdi Dadmehr

 

13. Selection of Structure-switching DNA Aptamers Binding Soluble Small Molecules and SPR Validation of Enrichment

Enrico Tenaglia and Carlotta Guiducci

 

14. Dielectrophoretic Stretching of DNA

Eva-Maria Laux, Frank F. Bier, and Ralph Hölzel

 

15. A Practical Guide to Molecular Dynamics Simulations of DNA Origami Systems

Jejoong Yoo, Chen-Yu Li , Scott Michael Slone, Chris Maffeo, and Aleksei Aksimentiev

 

16. Single-molecule Patterning via DNA Nanostructure Assembly: A Reusable Platform

Da Huang, Mark Freeley, and Matteo Palma

 

17. Directed Protein Adsorption through DNA Origami Masks

Saminathan Ramakrishnan, Guido Grundmeier, and Adrian Keller

 

18. DNA Origami Structures Interfaced to Inorganic Nanodevices

Claudia Dalmastri, Weihua Han, Stefano Vespucci, Liqian Wang, and Piero Morales

 

19. Tuning Gold Nanoparticles Plasmonic Properties by DNA Nanotechnology

Valentina Masciotti, Denys Naumenko, Marco Lazzarino, and Luca Piantanida

 

20. DNA-assisted Molecular Lithography

Boxuan Shen, Veikko Linko, and J. Jussi Toppari

 

21. Constructing Free Energy Landscapes of Nucleic Acid Hairpin Unfolding

Micah J. McCauley, Ioulia Rouzina, and Mark C. Williams 

Blurb

This second edition provides new and updated methods that detail new DNA nanotechnology techniques. Chapters focus on DNA origami nanostructures for arranging matter in the nanoscale or on their manipulation with the aid of other technologies, on procedures for making nucleic acids nanostructures of different kinds, and methods to simulate complex nanostructures or to use them in biosensing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, DNA Nanotechnology: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.