DNA Nanotechnology: Methods and Protocols

ISBN-13: 9781493961382 / Angielski / Miękka / 2016 / 361 str.

Giampaolo Zuccheri; Bruno Samori; Bruno Samor

Opis

Currently, nanotechnology is exposing the properties of DNA in unprecedented detail leading to new insights on the biological behavior and function of DNA. With the structural perfection of a self-assembling DNA nano-object, such as a DNA origami, it is clear how complex DNA is as a molecule, leading researchers to wonder how many different constructs could be designed and realized. DNA Nanotechnology: Methods and Protocols shows the procedures to follow in order to repeat methods that lead to such constructs or to the mastering of the characterization techniques used to study them. The chapters of this book are roughly divided into two parts: some cover the methods for preparing the nanostructures, from the rationale of the operations to the techniques for their handling, while other chapters deal more directly with advanced instrumental techniques that can manipulate and characterize molecules and nanostructures. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, DNA Nanotechnology: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and aims to ignite interest and spur activity in this young and rapidly growing research field. Includes cutting-edge methods and protocolsProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the experts

Spis treści

1. Synthesis and Characterization of Self-assembled DNA Nanostructures

            Chenxiang Lin, Yonggang Ke, Rahul Chhabra, Jaswinder Sharma, Yan Liu, and Hao Yan

 

2. Protocols for Self-assembly and Imaging of DNA Nanostructures

            Thomas L. Sobey and Friedrich C. Simmel

 

3. Self-Assembly of Metal-DNA Triangles and DNA Nanotubes with Synthetic Junctions

            Hua Yang, Pik Kwan Lo, Christopher K. McLaughlin, Graham D. Hamblin, Faisal A. Aldaye, and Hanadi F. Sleiman

 

4. DNA-Templated  Pd Conductive Metallic Nanowires

            Khoa Nguyen, Stephane Campidelli, and Arianna Filoramo

 

5. A Method to Map Spatiotemporal pH Changes Inside Living Cells using a pH Triggered DNA Nanoswitch

            Souvik Modi and Yamuna Krishnan

 

6. Control of Helical Handedness in DNA and PNA Nanostructures

            Roberto Corradini, Tullia Tedeschi, Stefano Sforza, Mark M. Green, and Rosangela Marchelli

 

7. G-quartet, G-quadruplex, and G-wire Regulated by Chemical Stimuli

            Daisuke Miyoshi and Naoki Sugimoto

 

8. Preparation and Atomic Force Microscopy of Quadruplex DNA

            James Vesenka

 

9. Synthesis of Long DNA-based Nanowires

            Alexander Kotlyar

 

10. G-wire Synthesis and Modification with Gold-nanoparticle

            Christian Leiterer, Andrea Csaki, and Wolfgang Fritzsche

 

11. Preparation of DNA Nanostructures with Repetitive Binding Motifs by Rolling Circle Amplification

            Edda Reiß, Ralph Hölzel, and Frank F. Bier

 

12. Controlled Confinement of DNA at the Nanoscale: Nanofabrication and Surface Bio-functionalization

            Matteo Palma, Justin Abramson, Alon A. Gorodetsky, Colin Nuckolls, Michael P. Sheetz, Shalom J. Wind, and James Hone

 

13. Templated Assembly of DNA Origami Gold Nanoparticle Arrays on Lithographically Patterned Surfaces

            Albert M. Hung and Jennifer N. Cha

 

14. DNA-modified Single Crystal and Nanoporous Silicon

            Andrew Houlton, Bernard A. Connolly, Andrew R. Pike, and Benjamin R. Horrocks

 

15. The Atomic Force Microscopy as a Lithographic Tool: Nanografting of DNA Nanostructures for Biosensing Applications

            Matteo Castronovo and Denis Scaini

 

16. Trapping and Immobilization of DNA Molecules Between Nanoelectrodes

            Anton Kuzyk, J. Jussi Toppari, and Päivi Törmä

 

17. DNA Contour Length Measurements as a Tool for the Structural Analysis of DNA and Nucleoprotein Complexes

            Claudio Rivetti

 

18. DNA Molecular Handles for Single Molecule Protein Folding Studies by Optical Tweezers

            Ciro Cecconi, Elizabeth A. Shank, Susan Marqusee, and Carlos Bustamante

 

19. Optimal Practices for Surface-tethered Single Molecule Total Internal Reflection Fluorescence Resonance Energy Transfer Analysis

            Matt V. Fagerburg and Sanford H. Leuba

 

20. Engineering Mononucleosomes for Single-Pair FRET Experiments

            Wiepke J.A. Koopmans, Ruth Buning, and John van Noort

 

21. Measuring DNA-Protein Binding Affinity on a Single Molecule Using Optical Tweezers

            Micah J. McCauley and Mark C. Williams

 

22. Modeling Nanopores for Sequencing DNA

            Jeffrey R. Comer, David B. Wells, and Aleksei Aksimentiev

Blurb

Currently, nanotechnology is exposing the properties of DNA in unprecedented detail leading to new insights on the biological behavior and function of DNA. With the structural perfection of a self-assembling DNA nano-object, such as a DNA origami, it is clear how complex DNA is as a molecule, leading researchers to wonder how many different constructs could be designed and realized. DNA Nanotechnology: Methods and Protocols shows the procedures to follow in order to repeat methods that lead to such constructs or to the mastering of the characterization techniques used to study them. The chapters of this book are roughly divided into two parts: some cover the methods for preparing the nanostructures, from the rationale of the operations to the techniques for their handling, while other chapters deal more directly with advanced instrumental techniques that can manipulate and characterize molecules and nanostructures. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, DNA Nanotechnology: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and aims to ignite interest and spur activity in this young and rapidly growing research field.