DNA Damage and Chromosomal Instability

ISBN-13: 9780443188985 / Twarda / 2024 / 318 str.

Spis treści

1. Induction of chromosome-specific micronuclei and chromothripsis by centromere inactivation
Peter Ly
2. Generation of aneuploid cells and assessment of their ability to survive in presence of chemotherapeutic agents
Stefano Santaguida
3. Genetic reporters to detect and quantify homologous recombination in yeast
Loraine Symington
4. Mapping histone variant genomic distribution: exploiting SNAP- Tag labelling to follow the dynamics of incorporation of H3 variants
Geneviève Almouzni and Jean-Pierre Quivy
5. Nascent DNA sequencing and its diverse applications in genome integrity research
Andre Nussenzweig and Jacob Paiano
6. Enrichment of DNA replication intermediates by EdU pull down
Ylli Doksani
7. DNA curtains for studying phase separation mechanisms of DNA-organizing proteins
Johannes Stigler
8. Visualizing the Dynamics of DNA Replication and Repair at the Single-Molecule Molecule Level
Gheorghe Chistol
9. Cloning and Expansion of Repetitive DNA Sequences
Gideon Coster and Sophie Lyn Williams
10. Using an ImageJ-based script to detect replication stress and associated cell cycle exit from G2 phase by fluorescence microscopy
Arne Lindqvist
11. An automated image analysis pipeline to quantify the coordination and overlap of transcription and replication activity in mammalian genomes
Stephan Hamperl
12. Monitoring Chk1 kinase activity dynamics in live single cell imaging assays
Olivier Gavet and Vicente Lebrec
13. Visualising DNA damage and repair using single molecule super resolution microscopy
Donna Whelan
14. Quantification of nuclear deformations and DNA damage foci dynamics by live-cell imaging
Francisca Lottersberger
15. Native FISH: A low- and high- throughput assay to analyze the Alternative Lengthening of Telomere (ALT) pathway
Eros Lazzerini-Denchi and Benura Azeroglu
16. Assessment of DNA Fibres
Chris Staples
17. A high-content flow cytometry and dual CRISPR-Cas9 based platform to quantify genetic interactions Priyanka Verma 18. Monitoring APOBEC3A protein levels in human cancer cells
John Maciejowski